Vitamin D-mediated regulation of CYP21A2 transcription — A novel mechanism for vitamin D action

• Published in: Biochimica et biophysica acta Vol. 1820; no. 10; pp. 1553 - 1559
• Main Authors: Lundqvist, Johan, Wikvall, Kjell, Norlin, Maria
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.10.2012
• Subjects: adrenal cortex; Animals; Basic Helix-Loop-Helix Transcription Factors - genetics; Basic Helix-Loop-Helix Transcription Factors - physiology; Calcitriol; Cells, Cultured; chromatin; CYP21A2; Farmaceutisk biokemi; gene expression; Gene Expression Regulation, Enzymologic - drug effects; gene silencing; Humans; luciferase; Mice; Models, Biological; mutagenesis; Pharmaceutical Biochemistry; precipitin tests; promoter regions; Promoter Regions, Genetic - drug effects; receptors; Receptors, Calcitriol - genetics; Receptors, Calcitriol - physiology; Retinoid X Receptors - genetics; Retinoid X Receptors - physiology; Signal Transduction - drug effects; Signal Transduction - genetics; small interfering RNA; Steroid; Steroid 21-Hydroxylase - genetics; Steroidogenesis; transcription (genetics); transcription factors; Transcription Factors - genetics; Transcription Factors - physiology; Transcription, Genetic - drug effects; Vitamin D; Vitamin D - analogs & derivatives; Vitamin D - pharmacology; Vitamin D - physiology; Vitamin D Response Element - drug effects; Vitamin D Response Element - physiology; Steroid; SF-1; Vitamin D; VDR; CYP21A2; WSTF; Calcitriol; VDRE; nVDRE; Steroidogenesis; RXR; VDIR

1α,25-Dihydroxyvitamin D3 has recently been reported to decrease expression and activity of CYP21A2. In this paper, we have studied the mechanisms for the 1α,25-dihydroxyvitamin D3-mediated effect on CYP21A2 transcriptional rate. We have studied the effects of 1α,25-dihydroxyvitamin D3 using lucifer...