CRISPR-Cas-based techniques for pathogen detection: Retrospect, recent advances, and future perspectives

• Published in: Journal of advanced research Vol. 50; pp. 69 - 82
• Main Authors: Huang, Tao, Zhang, Rui, Li, Jinming
• Format: Journal Article
• Language: English
• Published: Egypt Elsevier B.V 01.08.2023; Elsevier
• Subjects: CRISPR-Cas; CRISPR-Cas Systems; Nucleic Acids; Pathogen detection; Point-of-care testing; Polymerase Chain Reaction; Review; Standardized testing; Technology; Translations; Point-of-care testing; RPA; Ct values; HPV; rRT-PCR; SDA; SARS-CoV-2; SNP; SG; POCT; DENV; AuNPs; MRSA; CRISPR-Cas; crRNA; E. coli; ssDNA; NAATs; Pathogen detection; AP; ssRNA; ZIKV; Standardized testing; RCA; sgRNA; PC reporters; SMR; Mtb; LAMP; NGS; FC; PAM; TCEP; dsDNA; PCR; Qdots

[Display omitted] •Early pathogen detection is critical for disease treatment, prevention, and control.•Traditional pathogen detection techniques based on polymerase chain reaction are limited by the high cost of reagents and instruments, as well as the harsh technical operation.•The development of...