Oligosaccharide biosensor for direct monitoring of enzymatic activities using QCM-D

• Published in: Biosensors & bioelectronics Vol. 49; pp. 290 - 296
• Main Authors: Bouchet-Spinelli, Aurélie, Reuillard, Bertrand, Coche-Guérente, Liliane, Armand, Sylvie, Labbé, Pierre, Fort, Sébastien
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 15.11.2013; Elsevier
• Subjects: Bacillus - enzymology; biocompatible materials; bioengineering; biofuels; Biological and medical sciences; biomass; Biosensing Techniques - methods; Biosensors; Biotechnology; Carbohydrate biosensor; Carbohydrate-active enzyme; Carbohydrates; Colorimetry; enzyme activity; Enzyme Assays - methods; Enzymes; Fundamental and applied biological sciences. Psychology; Glycoside Hydrolases - analysis; Glycoside Hydrolases - metabolism; Glycosides; human health; Humans; hydrolases; metagenomics; Methods. Procedures. Technologies; Microbalances; Monitoring; Oligosaccharides; Oligosaccharides - chemistry; Oligosaccharides - metabolism; Quartz Crystal Microbalance Techniques - methods; Quartz-crystal microbalance with dissipation monitoring (QCM-D); Self-assembled monolayers; Various methods and equipments; Quartz-crystal microbalance with dissipation monitoring (QCM-D); Carbohydrate biosensor; Carbohydrate-active enzyme; Oligosaccharides; Self-assembled monolayers; Self assembly; Monolayer; Enzyme; Oligosaccharide; Crystals; Enzymatic activity; Biosensor; Carbohydrate; Monitoring; Quartz microbalance

Enzymatic modification of saccharidic biomass is a subject of intensive research with potential applications in plant or human health, design of biomaterials and biofuel production. Bioengineering and metagenomics provide access to libraries of glycoside hydrolases but the biochemical characterizati...