3D Correlative Cryo-Structured Illumination Fluorescence and Soft X-ray Microscopy Elucidates Reovirus Intracellular Release Pathway

• Published in: Cell Vol. 182; no. 2; pp. 515 - 530.e17
• Main Authors: Kounatidis, Ilias, Stanifer, Megan L., Phillips, Michael A., Paul-Gilloteaux, Perrine, Heiligenstein, Xavier, Wang, Hongchang, Okolo, Chidinma A., Fish, Thomas M., Spink, Matthew C., Stuart, David I., Davis, Ilan, Boulant, Steeve, Grimes, Jonathan M., Dobbie, Ian M., Harkiolaki, Maria
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 23.07.2020; Elsevier; Cell Press
• Subjects: Bioengineering; Cell Line, Tumor; CLXT; computed tomography; Computer Science; Computer Vision and Pattern Recognition; correlative imaging; Cryoelectron Microscopy - instrumentation; Cryoelectron Microscopy - methods; Endosomes - metabolism; Endosomes - virology; fluorescence; fluorescence microscopy; Fluorescent Dyes - chemistry; Humans; ice; image analysis; Imaging; Imaging, Three-Dimensional; Life Sciences; lighting; Microscopy, Fluorescence; Reoviridae; Reoviridae - chemistry; Reoviridae - physiology; reovirus biology; structured illumination microscopy; Virus Release - physiology; X-radiation; X-ray tomography; X-ray tomography; reovirus biology; CLXT; structured illumination microscopy; correlative imaging

Imaging of biological matter across resolution scales entails the challenge of preserving the direct and unambiguous correlation of subject features from the macroscopic to the microscopic level. Here, we present a correlative imaging platform developed specifically for imaging cells in 3D under cry...