Identification of a Chloroplast Ribonucleoprotein Complex Containing Trans-splicing Factors, Intron RNA, and Novel Components

• Published in: Molecular & cellular proteomics Vol. 12; no. 7; pp. 1912 - 1925
• Main Authors: Jacobs, Jessica, Marx, Christina, Kock, Vera, Reifschneider, Olga, Fränzel, Benjamin, Krisp, Christoph, Wolters, Dirk, Kück, Ulrich
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.07.2013; The American Society for Biochemistry and Molecular Biology
• Subjects: Algal Proteins - chemistry; Algal Proteins - metabolism; Chlamydomonas reinhardtii; Chlamydomonas reinhardtii - genetics; Chlamydomonas reinhardtii - metabolism; Chloroplasts - genetics; Chloroplasts - metabolism; Introns; Protein Interaction Maps; Ribonucleoproteins - metabolism; RNA Precursors - genetics; RNA, Plant - genetics; Trans-Splicing

Maturation of chloroplast psaA pre-mRNA from the green alga Chlamydomonas reinhardtii requires the trans-splicing of two split group II introns. Several nuclear-encoded trans-splicing factors are required for the correct processing of psaA mRNA. Among these is the recently identified Raa4 protein, w...