DNA Topoisomerase II Mutant of Saccharomyces cerevisiae: Topoisomerase II is Required for Segregation of Daughter Molecules at the Termination of DNA Replication

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 81; no. 9; pp. 2616 - 2620
• Main Authors: DiNardo, Stephen, Voelkel, Karen, Sternglanz, Rolf
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 01.05.1984; National Acad Sciences
• Subjects: Cell Cycle; Chromosomes; Dimers; DNA; DNA Replication; DNA Topoisomerases, Type I - genetics; Molecules; Monomers; Mother cells; Mutation; Nucleic Acid Conformation; Phenotype; Phenotypes; Plasmids; Saccharomyces cerevisiae - genetics; Yeasts

A temperature-sensitive DNA topoisomerase II mutant of the yeast Saccharomyces cerevisiae has been identified. Genetic analysis shows that a single recessive nuclear mutation is responsible for both temperature-sensitive growth and enzymatic activity. Thus, topoisomerase II is essential for viabilit...