Tyrosylprotein sulfotransferase suppresses ABA signaling via sulfation of SnRK2.2/2.3/2.6

• Published in: Journal of integrative plant biology Vol. 65; no. 8; pp. 1846 - 1851
• Main Authors: Wang, Jun, Wang, Chunyan, Wang, Tianrun, Zhang, Shizhong, Yan, Kang, Yang, Guodong, Wu, Changai, Zheng, Chengchao, Huang, Jinguang
• Format: Journal Article
• Language: English
• Published: China (Republic : 1949- ) Wiley Subscription Services, Inc 01.08.2023; State Key Laboratory of Crop Biology,College of Life Sciences,Shandong Agricultural University,Taian 271018,China; Department of Food Science and Engineering,Shandong Agriculture and Engineering University,Jinan 250100,China%State Key Laboratory of Crop Biology,College of Life Sciences,Shandong Agricultural University,Taian 271018,China
• Subjects: ABA signaling; Abscisic acid; Arabidopsis; Desensitization; Genetic screening; growth and development; mutants; Plant growth; Plant hormones; post-translational modification; Proteins; SnRK2.2/2.3/2.6; stress tolerance; sulfates; Sulfation; Sulfotransferase; Tyrosine; tyrosylprotein sulfotransferase; ABA signaling; tyrosylprotein sulfotransferase; sulfation; SnRK2.2/2.3/2.6; Arabidopsis; tyrosylprotein sulfo-transferase
• ISSN: 1672-9072; 1744-7909; 1744-7909
• DOI: 10.1111/jipb.13493

SUMMARY Phytohormone abscisic acid (ABA) plays vital roles in stress tolerance, while long‐term overactivation of ABA signaling suppresses plant growth and development. However, the braking mechanism of ABA responses is not clear. Protein tyrosine sulfation catalyzed by tyrosylprotein sulfotransferase (TPST) is a critical post‐translational modification. Through genetic screening, we identified a tpst mutant in Arabidopsis that was hypersensitive to ABA. In‐depth analysis revealed that TPST could interact with and sulfate SnRK2.2/2.3/2.6, which accelerated their degradation and weakened the ABA signaling. Taken together, these findings uncovered a novel mechanism of desensitizing ABA responses via protein sulfation. Tyrosylprotein sulfotransferase alleviates the side effects of abscisic acid responses by sulfating the kinases SnRK2.2, SnRK2.3, and SnRK2.6, and accelerating their degradation.