Profiling of mouse macrophage lipidome using direct infusion shotgun mass spectrometry

• Published in: STAR protocols Vol. 2; no. 1; p. 100235
• Main Authors: Hsieh, Wei-Yuan, Williams, Kevin J., Su, Baolong, Bensinger, Steven J.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 19.03.2021; Elsevier
• Subjects: Animals; Cell culture; High Throughput Screening; Immunology; Lipid Metabolism; Lipidomics; Macrophages - metabolism; Mass Spectrometry; Metabolism; Mice; Model Organisms; Protocol; High Throughput Screening; Cell culture; Model Organisms; Immunology; Metabolism; Mass Spectrometry

Immune cells, such as macrophages, reprogram their lipid metabolism in response to the activation of pattern recognition receptors (e.g., TLRs, NLRs) and cytokine receptors (e.g., interferons, interleukins). Profiling these changes can be achieved with shotgun mass spectrometry. This protocol provid...