Sliced Human Cortical Organoids for Modeling Distinct Cortical Layer Formation

• Published in: Cell stem cell Vol. 26; no. 5; pp. 766 - 781.e9
• Main Authors: Qian, Xuyu, Su, Yijing, Adam, Christopher D., Deutschmann, Andre U., Pather, Sarshan R., Goldberg, Ethan M., Su, Kenong, Li, Shiying, Lu, Lu, Jacob, Fadi, Nguyen, Phuong T.T., Huh, Sooyoung, Hoke, Ahmet, Swinford-Jackson, Sarah E., Wen, Zhexing, Gu, Xiaosong, Pierce, R. Christopher, Wu, Hao, Briand, Lisa A., Chen, H. Isaac, Wolf, John A., Song, Hongjun, Ming, Guo-li
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 07.05.2020
• Subjects: Brain organoid; cerebral cortex; DISC1; forebrain organoid; human iPSC; Humans; Induced Pluripotent Stem Cells; lamination; Neocortex; neurodevelopment; Neurogenesis; neuron fate specification; Neurons; Organoids; schizophrenia; WNT; cerebral cortex; forebrain organoid; lamination; neurodevelopment; Brain organoid; neuron fate specification; human iPSC; schizophrenia; DISC1; WNT

Human brain organoids provide unique platforms for modeling development and diseases by recapitulating the architecture of the embryonic brain. However, current organoid methods are limited by interior hypoxia and cell death due to insufficient surface diffusion, preventing generation of architectur...