Toll-Like Receptors Induce Signal-Specific Reprogramming of the Macrophage Lipidome

• Published in: Cell metabolism Vol. 32; no. 1; pp. 128 - 143.e5
• Main Authors: Hsieh, Wei-Yuan, Zhou, Quan D., York, Autumn G., Williams, Kevin J., Scumpia, Philip O., Kronenberger, Eliza B., Hoi, Xen Ping, Su, Baolong, Chi, Xun, Bui, Viet L., Khialeeva, Elvira, Kaplan, Amber, Son, Young Min, Divakaruni, Ajit S., Sun, Jie, Smale, Stephen T., Flavell, Richard A., Bensinger, Steven J.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 07.07.2020
• Subjects: acetylated-LDL; Animals; Cell Line; host defense; inflammation; interferon; Lipidomics; macrophages; Macrophages - metabolism; Male; Mice; Mice, Knockout; Mice, Transgenic; MyD88; Signal Transduction; stable isotope tracer analysis; stearoyl-CoA desaturase; toll-like receptors; Toll-Like Receptors - metabolism; macrophages; toll-like receptors; stable isotope tracer analysis; inflammation; stearoyl-CoA desaturase; host defense; acetylated-LDL; interferon; MyD88; lipidomics

Macrophages reprogram their lipid metabolism in response to activation signals. However, a systems-level understanding of how different pro-inflammatory stimuli reshape the macrophage lipidome is lacking. Here, we use complementary “shotgun” and isotope tracer mass spectrometry approaches to define...