Effect of Type I Antifreeze Proteins on the Freezing and Melting Processes of Cryoprotective Solutions Studied by Site-Directed Spin Labeling Technique

• Published in: Crystals (Basel) Vol. 9; no. 7; p. 352
• Main Authors: Perez, Adiel F., Taing, Kyle R., Quon, Justin C., Flores, Antonia, Ba, Yong
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01.07.2019
• Subjects: antifreeze protein; Cooling; cryo-photo microscopy; Cryopreservation; cryoprotective agent; Electron paramagnetic resonance; Freezing; Glycerol; Hemodialysis; ice crystal; Ice crystals; Ice formation; Labeling; Low temperature; Melting points; Nitrogen; Organs; Peptides; Propylene; Proteins; Room temperature; spin labeling; United States > US

Antifreeze proteins (AFPs) protect organisms living in subzero environments from freezing injury, which render them potential applications for cryopreservation of living cells, organs, and tissues. Cryoprotective agents (CPAs), such as glycerol and propylene glycol, have been used as ingredients to...