Cyclic uniaxial cell stretching in tissue culture using a LEGO®-based mechanical stretcher and a polydimethylsiloxane stretchable vessel

• Published in: STAR protocols Vol. 2; no. 2; p. 100437
• Main Authors: Boulter, Etienne, Féral, Chloé C.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 18.06.2021; Elsevier
• Subjects: Biomechanical Phenomena - physiology; Biophysics; Cell Biology; Cell Culture Techniques - instrumentation; Cell Culture Techniques - methods; Cells, Cultured - cytology; Cells, Cultured - physiology; Dimethylpolysiloxanes - chemistry; Equipment Design; Fibroblasts - cytology; Fibroblasts - physiology; HeLa Cells; Humans; Protocol; Stress, Mechanical; Tissue Culture Techniques - instrumentation; Tissue Culture Techniques - methods; Biophysics; Cell Biology

Mechanical signals are essential for the regulation of many biological processes. Therefore, it has become paramount to account for these mechanical parameters when exploring biological processes. Here, we describe a protocol to apply cyclic uniaxial stretch on cells in culture using a LEGO®-based m...