Local irradiation of patient-derived tumors in immunodeficient mice
Severe combined immunodeficient mice are typically used for xenografting experiments and show reliable tumor engraftment; however, their Prkdscid mutation renders them highly sensitive to irradiation. Here, we describe a protocol that allows safe local irradiation of tumor xenografts in immunodefici...
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Published in | STAR protocols Vol. 4; no. 1; p. 102098 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
17.03.2023
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | Severe combined immunodeficient mice are typically used for xenografting experiments and show reliable tumor engraftment; however, their Prkdscid mutation renders them highly sensitive to irradiation. Here, we describe a protocol that allows safe local irradiation of tumor xenografts in immunodeficient mice. We detail the steps for the establishment and handling of patient-derived cancer cultures, subcutaneous injection of cancer cells on the mouse hind limb, localized irradiation in mice, tumor monitoring, and tumor characterization via histological and immunohistochemical assessment.
For complete details on the use and execution of this protocol, please refer to Dings et al. (2022).1
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•Protocol for local irradiation of tumor xenografts in SCID mice•Steps for dissociation of patient-derived tissues and establishment of cancer cultures•Subcutaneous injection of patient-derived cells on mouse hind limb and tumor monitoring•Can be widely used for safe irradiation of tumors in immunodeficient mice
Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
Severe combined immunodeficient mice are typically used for xenografting experiments and show reliable tumor engraftment; however, their Prkdscid mutation renders them highly sensitive to irradiation. Here, we describe a protocol that allows safe local irradiation of tumor xenografts in immunodeficient mice. We detail the steps for the establishment and handling of patient-derived cancer cultures, subcutaneous injection of cancer cells on the mouse hind limb, localized irradiation in mice, tumor monitoring, and tumor characterization via histological and immunohistochemical assessment. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Senior authors Technical contact Lead contact |
ISSN: | 2666-1667 2666-1667 |
DOI: | 10.1016/j.xpro.2023.102098 |