High-Efficiency Multi-site Genomic Editing of Pseudomonas putida through Thermoinducible ssDNA Recombineering

• Published in: iScience Vol. 23; no. 3; p. 100946
• Main Authors: Aparicio, Tomas, Nyerges, Akos, Martínez-García, Esteban, de Lorenzo, Víctor
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 27.03.2020; Elsevier
• Subjects: Bioengineering; Biotechnology; Metabolic Engineering; Microbial Biotechnology; Bioengineering; Metabolic Engineering; Biotechnology; Microbial Biotechnology

Application of single-stranded DNA recombineering for genome editing of species other than enterobacteria is limited by the efficiency of the recombinase and the action of endogenous mismatch repair (MMR) systems. In this work we have set up a genetic system for entering multiple changes in the chro...