Generation of Inexpensive, Highly Labeled Probes for Fluorescence In Situ Hybridization (FISH)

• Published in: STAR protocols Vol. 1; no. 1; p. 100006
• Main Authors: Sharma, Rahul, Meister, Peter
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 19.06.2020; Elsevier
• Subjects: Animals; Humans; In Situ Hybridization, Fluorescence - methods; Mice; Molecular Probes - chemistry; Molecular Probes - metabolism; Polymerase Chain Reaction - methods; Protocol

DNA-FISH remains the method of choice to visualize genomic regions in situ ranging from a single locus to entire chromosomes. Current methods to generate probes rely on expensive kits that vary in labeling efficiency and are limited by the size and/or amount of starting material and by the choice of...