Integrated, Step-Wise, Mass-Isotopomeric Flux Analysis of the TCA Cycle

• Published in: Cell metabolism Vol. 22; no. 5; pp. 936 - 947
• Main Authors: Alves, Tiago C., Pongratz, Rebecca L., Zhao, Xiaojian, Yarborough, Orlando, Sereda, Sam, Shirihai, Orian, Cline, Gary W., Mason, Graeme, Kibbey, Richard G.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 03.11.2015
• Subjects: Acetyl Coenzyme A - metabolism; Animals; Carbon Isotopes; Citrates - metabolism; Citric Acid - metabolism; Citric Acid Cycle - genetics; Insulin - genetics; Insulin - metabolism; Isocitrate Dehydrogenase - genetics; Isocitrate Dehydrogenase - metabolism; Oxaloacetic Acid - metabolism; Oxidation-Reduction; Oxygen Consumption; Pyruvate Carboxylase - genetics; Pyruvate Carboxylase - metabolism; Pyruvate Dehydrogenase Complex - genetics; Pyruvate Dehydrogenase Complex - metabolism; Pyruvic Acid - metabolism; Rats

Mass isotopomer multi-ordinate spectral analysis (MIMOSA) is a step-wise flux analysis platform to measure discrete glycolytic and mitochondrial metabolic rates. Importantly, direct citrate synthesis rates were obtained by deconvolving the mass spectra generated from [U-13C6]-D-glucose labeling for...