Comparing Methods for Identifying Bacillus Strains Capable of Producing the Antifungal Lipopeptide Iturin A

• Published in: Current microbiology Vol. 56; no. 1; pp. 1 - 5
• Main Authors: Hsieh, Feng-Chia, Lin, Tsung-Chun, Meng, Menghsiao, Kao, Suey-Sheng
• Format: Journal Article
• Language: English
• Published: New York New York : Springer-Verlag 01.01.2008; Springer-Verlag; Springer Nature B.V
• Subjects: Acyl-Carrier Protein S-Malonyltransferase; Acyl-Carrier Protein S-Malonyltransferase - genetics; Antibiotics; Antifungal Agents; Antifungal Agents - isolation & purification; Antifungal Agents - metabolism; Bacillus; Bacillus (bacteria); Bacillus - chemistry; Bacillus - genetics; Bacillus - isolation & purification; Bacillus - metabolism; Bacillus spp; Bacterial Proteins; Bacterial Proteins - genetics; Biomedical and Life Sciences; biosynthesis; Biotechnology; chemistry; Chromatography, High Pressure Liquid; Chromatography, High Pressure Liquid - methods; DNA, Bacterial; DNA, Bacterial - genetics; genetics; growth retardation; high performance liquid chromatography; isolation & purification; iturin; Iturin A; Life Sciences; lipopeptides; Liquid chromatography; metabolism; Metabolites; methods; microbial growth; Microbial Sensitivity Tests; Microbial Sensitivity Tests - methods; Microbiology; Peptides; Peptides, Cyclic; Peptides, Cyclic - biosynthesis; Peptides, Cyclic - genetics; Peptides, Cyclic - isolation & purification; Polymerase chain reaction; Polymerase Chain Reaction - methods; screening; Secondary metabolites; Transferases (Other Substituted Phosphate Groups); Transferases (Other Substituted Phosphate Groups) - genetics; Yeasts; Polymerase chain reaction; Iturin A; spp

Lipopeptides represent a unique class of bioactive microbial secondary metabolites, and iturin A shows attractive antibiotic properties among them. This study compares three methods, such as yeast/fungal growth inhibition assay, quantitative high-performance liquid chromatography (HPLC) and polymera...