A Multiplexed Single-Cell CRISPR Screening Platform Enables Systematic Dissection of the Unfolded Protein Response

• Published in: Cell Vol. 167; no. 7; pp. 1867 - 1882.e21
• Main Authors: Adamson, Britt, Norman, Thomas M., Jost, Marco, Cho, Min Y., Nuñez, James K., Chen, Yuwen, Villalta, Jacqueline E., Gilbert, Luke A., Horlbeck, Max A., Hein, Marco Y., Pak, Ryan A., Gray, Andrew N., Gross, Carol A., Dixit, Atray, Parnas, Oren, Regev, Aviv, Weissman, Jonathan S.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.12.2016
• Subjects: Animals; cell-to-cell heterogeneity; Clustered Regularly Interspaced Short Palindromic Repeats; CRIPSRi; CRISPR; Endoribonucleases; Feedback; genome-scale screening; Humans; Models, Molecular; Protein Serine-Threonine Kinases; RNA, Guide, CRISPR-Cas Systems - metabolism; Sequence Analysis, RNA - methods; Single-Cell Analysis - methods; single-cell genomics; Single-cell RNA-seq; Transcription, Genetic; Unfolded Protein Response; CRISPR; unfolded protein response; Single-cell RNA-seq; cell-to-cell heterogeneity; single-cell genomics; genome-scale screening; CRIPSRi

Functional genomics efforts face tradeoffs between number of perturbations examined and complexity of phenotypes measured. We bridge this gap with Perturb-seq, which combines droplet-based single-cell RNA-seq with a strategy for barcoding CRISPR-mediated perturbations, allowing many perturbations to...