Multichromosomal Genome Structure and Confirmation of Diazotrophy in Novel Plant-Associated Burkholderia Species

• Published in: Applied and Environmental Microbiology Vol. 74; no. 14; pp. 4574 - 4579
• Main Authors: Martínez-Aguilar, Lourdes, Díaz, Rafael, Peña-Cabriales, Juan José, Estrada-de los Santos, Paulina, Dunn, Michael F, Caballero-Mellado, Jesús
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.07.2008; American Society for Microbiology (ASM)
• Subjects: Base Sequence; Biological and medical sciences; Burkholderia; Burkholderia - classification; Burkholderia - enzymology; Burkholderia - genetics; Chromosomes; Chromosomes, Bacterial - genetics; DNA, Bacterial - isolation & purification; Electrophoresis, Gel, Pulsed-Field; Evolutionary and Genomic Microbiology; Flowers & plants; Fundamental and applied biological sciences. Psychology; Genome, Bacterial; Genomics; Isotopes; Microbiology; Molecular Sequence Data; Nitrogen Fixation; Nucleic Acid Hybridization; Oxidoreductases - genetics; Phylogeny; Plants - microbiology; Polymerase Chain Reaction; Ribonucleic acid; RNA; RNA, Ribosomal, 16S - isolation & purification; Sequence Analysis, DNA; Soil Microbiology; Bacteria; Burkholderia; Genome; Structure; Diazotrophy

Pulsed-field gel electrophoresis and 16S rRNA hybridization experiments showed that multichromosome genome structures and very large genome sizes (6.46 to 8.73 Mb) are prevalent in novel plant-associated Burkholderia species. ¹⁵N₂ isotope dilution assays revealed unambiguous diazotrophy in these nov...