Peroxidase from foxtail millet bran exerts anti-colorectal cancer activity via targeting cell-surface GRP78 to inactivate STAT3 pathway

• Published in: Acta pharmaceutica Sinica. B Vol. 12; no. 3; pp. 1254 - 1270
• Main Authors: Shan, Shuhua, Niu, Jinping, Yin, Ruopeng, Shi, Jiangying, Zhang, Lizhen, Wu, Caihong, Li, Hanqing, Li, Zhuoyu
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.03.2022; Elsevier
• Subjects: Colorectal cancer; csGRP78; FMBP; Foxtail millet bran; Original; ROS; STAT3; FDA; Colorectal cancer; SPF; EGFR; CETSA; NBD; SBD; ISM; csGRP78; rGRP78; TRAIL; CAC; H&E; STAT3; GRP78; Co-IP; Foxtail millet bran; ER; DCFH-DA; MPs; FMBP; CRC; CDKs; ROS; PD-1; FMBP, peroxidase derived from foxtail millet bran; CDKs, cyclin-dependent kinases; GRP78, glucose-regulated protein 78; SPF, specific pathogen free; TRAIL, tumor necrosis factor-related apoptosis-inducing ligand; CRC, colorectal cancer; H&E, hematoxylin & eosin; ROS, reactive oxygen species; CAC, colitis-associated carcinogenesis; DCFH-DA, dichloro-dihydro-fluorescein diacetate; SBD, substrate-binding domain of csGRP78; Co-IP, co-immunoprecipitation; FDA, U.S. Food and Drug Administration; PD-1, programmed death-1; STAT3, signal transducer and activator of transcription 3; NBD, the nucleotide binding domain of csGRP78; EGFR, epidermal growth factor receptor; CETSA, cellular thermal shift assay; ISM, isthmin; MPs, membrane proteins; csGRP78, cell surface glucose-regulated protein 78; rGRP78, recombinant GRP78; ER, endoplasmic reticulum

Molecular targeted therapy has become an emerging promising strategy in cancer treatment, and screening the agents targeting at cancer cell specific targets is very desirable for cancer treatment. Our previous study firstly found that a secretory peroxidase of class III derived from foxtail millet b...