Polymerase chain reaction-free detection of hepatitis B virus DNA using a nanostructured impedance biosensor

• Published in: Biosensors & bioelectronics Vol. 77; pp. 603 - 608
• Main Authors: Chen, Chun-Cheng, Lai, Zi-Lun, Wang, Gou-Jen, Wu, Chun-Ying
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 15.03.2016
• Subjects: Aluminum oxide; Barrier layers; Base Sequence; Biosensing Techniques - instrumentation; Biosensors; Conductometry - instrumentation; Deoxyribonucleic acid; Detection; detection limit; DNA; DNA viruses; DNA, Viral - analysis; DNA, Viral - genetics; electrochemistry; Electrodes; Equipment Design; Equipment Failure Analysis; genes; Gold; HBV DNA; Hepatitis B virus; Hepatitis B virus - genetics; Hepatitis B virus - isolation & purification; Molecular Sequence Data; nanogold; Nanostructure; Nanostructured impedance biosensor; Nanotechnology - instrumentation; nucleotide sequences; PCR-free detection; Polymerase Chain Reaction; Reproducibility of Results; Sensitivity and Specificity; Sequence Analysis, DNA - instrumentation; HBV DNA; Nanostructured impedance biosensor; PCR-free detection

A polymerase chain reaction (PCR)-free technique for the effective detection of genomic length hepatitis B virus (HBV) DNA is described in this study. The honeycomb-like barrier layer of an anodic aluminum oxide (AAO) film having a uniform nanohemisphere array was used as the substrate of the sensin...