The comparison of ZFNs, TALENs, and SpCas9 by GUIDE-seq in HPV-targeted gene therapy

• Published in: Molecular therapy. Nucleic acids Vol. 26; pp. 1466 - 1478
• Main Authors: Cui, Zifeng, Liu, Hui, Zhang, Hongfeng, Huang, Zhaoyue, Tian, Rui, Li, Lifang, Fan, Weiwen, Chen, Yili, Chen, Lijie, Zhang, Sen, Das, Bhudev C., Severinov, Konstantin, Hitzeroth, Inga Isabel, Debata, Priya Ranjan, Jin, Zhuang, Liu, Jiashuo, Huang, Zheying, Xie, Weiling, Xie, Hongxian, Lang, Bin, Ma, Ji, Weng, Haiyan, Tian, Xun, Hu, Zheng
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 03.12.2021; American Society of Gene & Cell Therapy; Elsevier
• Subjects: CRISPR; GUIDE-seq; HPV gene therapy; Original; TALEN; ZFN; CRISPR; HPV gene therapy; ZFN; TALEN; GUIDE-seq

Zinc-finger nucleases (ZFNs), transcription activator-like endonucleases (TALENs), and CRISPR-associated Cas9 endonucleases are three major generations of genome editing tools. However, no parallel comparison about the efficiencies and off-target activity of the three nucleases has been reported, wh...