A FACS-Based Genome-wide CRISPR Screen Reveals a Requirement for COPI in Chlamydia trachomatis Invasion

• Published in: iScience Vol. 11; pp. 71 - 84
• Main Authors: Park, Joseph S., Helble, Jennifer D., Lazarus, Jacob E., Yang, Guanhua, Blondel, Carlos J., Doench, John G., Starnbach, Michael N., Waldor, Matthew K.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 25.01.2019; Elsevier
• Subjects: Cell Biology; Genetic Engineering; Genetic Screens; Host-pathogen Interactions; Medical Microbiology; Methodology in Biological Sciences; Molecular Mechanism of Behavior; Molecular Microbiology; Medical Microbiology; Host-pathogen Interactions; Genetic Engineering; Molecular Mechanism of Behavior; Genetic Screens; Methodology in Biological Sciences; Cell Biology; Molecular Microbiology

The invasion of Chlamydia trachomatis, an obligate intracellular bacterium, into epithelial cells is driven by a complex interplay of host and bacterial factors. To comprehensively define the host genes required for pathogen invasion, we undertook a fluorescence-activated cell sorting (FACS)-based C...