Autophagy enhanced by curcumin ameliorates inflammation in atherogenesis via the TFEB–P300–BRD4 axis

• Published in: Acta pharmaceutica Sinica. B Vol. 12; no. 5; pp. 2280 - 2299
• Main Authors: Li, Xuesong, Zhu, Ruigong, Jiang, Hong, Yin, Quanwen, Gu, Jiaming, Chen, Jiajing, Ji, Xian, Wu, Xuan, Fu, Haiping, Wang, Hui, Tang, Xin, Gao, Yuanqing, Wang, Bingjian, Ji, Yong, Chen, Hongshan
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.05.2022; Elsevier
• Subjects: Atherosclerosis; Autophagy; BRD4; Curcumin; Inflammation; Macrophage; Original; P300; TFEB; Acetyl-H3; MCP-1; P300; HFD; ChIP; Autophagy; ORO; siRNAs; SE; Re-ChIP; Atherosclerosis; FCs; Curcumin; BRD4; ATG5; Cur; qRT-PCR; TFEB; Inflammation; ox-LDL; IL-1β; CQ; BET; NAC; Dil-ox-LDL; LIR; TNF-α; ROS; CVDs; mTORC1; Macrophage; IL-1β, interleukin 1β; TNF-α, tumor necrosis factor α; ChIP, chromatin immunoprecipitation; SE, super-enhancer; Re-ChIP, re-chromatin immunoprecipitation; BET, bromodomain and extra-terminal; TFEB, transcription factor EB; qRT-PCR, quantitative real-time polymerase chain reaction; HFD, high-fat diet; Dil-ox-LDL, 1,1′-dioctadecyl-3,3,3′,3′-tetramethy-lindocarbocyanine perchlorate labeled oxidized low-density lipoprotein; ATG5, autophagy-related 5; ROS, reactive oxygen species; NAC, N-acetyl-l-cysteine; ox-LDL, oxidized low-density lipoprotein; BRD4, bromodomain protein 4; MCP-1, monocyte chemotactic protein 1; Acetyl-H3, acetyl-histone 3; CQ, chloroquine; siRNAs, small interference RNAs; mTORC1, mammalian target of rapamycin complex 1; ORO, Oil red O; LIR, LC3-interacting region; Cur, curcumin; CVDs, cardiovascular diseases; FCs, foam cells

Disturbance of macrophage-associated lipid metabolism plays a key role in atherosclerosis. Crosstalk between autophagy deficiency and inflammation response in foam cells (FCs) through epigenetic regulation is still poorly understood. Here, we demonstrate that in macrophages, oxidized low-density lip...