Optimized protocol to isolate primary mouse peritoneal macrophage metabolites

• Published in: STAR protocols Vol. 3; no. 4; p. 101668
• Main Authors: De Jesus, Adam, Pusec, Carolina M., Nguyen, Tivoli, Keyhani-Nejad, Farnaz, Gao, Peng, Weinberg, Samuel E., Ardehali, Hossein
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 16.12.2022; Elsevier
• Subjects: Animals; Cell isolation; Glucose; Immunology; Macrophages, Peritoneal; Mass spectrometry; Metabolism; Metabolomics; Metabolomics - methods; Methanol; Mice; Protocol; Metabolomics; Immunology; Metabolism; Cell isolation; Mass spectrometry

Peritoneal macrophages (PMs) have been shown to have higher stability compared to other macrophage subtypes. However, obtaining enough PMs from a single mouse is often a limitation for metabolomics analysis. Here, we describe a protocol to isolate metabolites from a small number of mouse primary PMs...