Tracking and characterization of partial and full epithelial-mesenchymal transition cells in a mouse model of metastatic breast cancer

• Published in: STAR protocols Vol. 3; no. 2; p. 101438
• Main Authors: Lüönd, Fabiana, Santacroce, Natascha, Beisel, Christian, Guérard, Laurent, Bürglin, Thomas R., Christofori, Gerhard, Sugiyama, Nami
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 17.06.2022; Elsevier
• Subjects: Cancer; Cell Biology; Cell Differentiation; Flow Cytometry/Mass Cytometry; Microscopy; Molecular Biology; Protocol; RNAseq; Single Cell; Microscopy; Molecular Biology; Flow Cytometry/Mass Cytometry; Single Cell; Cell Differentiation; RNAseq; Cell Biology; Cancer

The various stages of epithelial-mesenchymal transition (EMT) generate phenotypically heterogeneous populations of cells. Here, we detail a dual recombinase lineage tracing system using a transgenic mouse model of metastatic breast cancer to trace and characterize breast cancer cells at different EM...