Hematopoietic Stem Cell Arrival Triggers Dynamic Remodeling of the Perivascular Niche

• Published in: Cell Vol. 160; no. 1-2; pp. 241 - 252
• Main Authors: Tamplin, Owen J., Durand, Ellen M., Carr, Logan A., Childs, Sarah J., Hagedorn, Elliott J., Li, Pulin, Yzaguirre, Amanda D., Speck, Nancy A., Zon, Leonard I.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.01.2015
• Subjects: Animals; Animals, Genetically Modified; Cell Division; Core Binding Factor alpha Subunits - genetics; Core Binding Factor alpha Subunits - metabolism; Embryo, Nonmammalian - blood supply; Embryo, Nonmammalian - physiology; Endothelium - cytology; Endothelium - physiology; Hematopoietic Stem Cells - cytology; Hematopoietic Stem Cells - physiology; Mesoderm - cytology; Mesoderm - metabolism; Mice; Mice, Inbred C57BL; Stem Cell Niche; Stromal Cells - cytology; Stromal Cells - metabolism; Zebrafish - embryology; Zebrafish - physiology

Hematopoietic stem and progenitor cells (HSPCs) can reconstitute and sustain the entire blood system. We generated a highly specific transgenic reporter of HSPCs in zebrafish. This allowed us to perform high-resolution live imaging on endogenous HSPCs not currently possible in mammalian bone marrow....