Target-catalyzed hairpin assembly and intramolecular/intermolecular co-reaction for signal amplified electrochemiluminescent detection of microRNA

• Published in: Biosensors & bioelectronics Vol. 77; pp. 442 - 450
• Main Authors: Yu, Yan-Qing, Wang, Ji-Peng, Zhao, Min, Hong, Lin-Ru, Chai, Ya-Qin, Yuan, Ruo, Zhuo, Ying
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 15.03.2016
• Subjects: Amplification; Assembly; Base Sequence; biosensors; Carbon; Catalysis; Conductometry - instrumentation; detection limit; DNA probes; Electrochemiluminescence; Electrodes; Equipment Design; Equipment Failure Analysis; Gold; Graphene; graphene oxide; Intermolecular co-reaction; Intramolecular co-reaction; Inverted Repeat Sequences - genetics; Luminescent Measurements - instrumentation; microRNA; microRNA-21; MicroRNAs - analysis; MicroRNAs - chemistry; MicroRNAs - genetics; Molecular Sequence Data; nanocarriers; nanogold; Nanostructure; ruthenium; Semicarbazides - chemistry; Strategy; Target recycle; Intermolecular co-reaction; Electrochemiluminescence; Target recycle; microRNA-21; Intramolecular co-reaction

Herein, a new electrochemiluminescence (ECL) strategy for enzyme-free microRNA-21 (miR-21) amplified detection was designed based on target-catalyzed hairpin assembly by combining the signal-amplification capability of both intramolecular and intermolecular ECL co-reaction. In this strategy, two hai...