Gastric digestion of α-lactalbumin in adult human subjects using capsule endoscopy and nasogastric tube sampling

• Published in: British journal of nutrition Vol. 112; no. 4; pp. 638 - 646
• Main Authors: Sullivan, Louise M., Kehoe, Joseph J., Barry, Lillian, Buckley, Martin J. M., Shanahan, Fergus, Mok, K. H., Brodkorb, André
• Format: Journal Article
• Language: English
• Published: Cambridge, UK Cambridge University Press 28.08.2014
• Subjects: Adult; adults; Animals; Antineoplastic Agents - pharmacology; beverages; Biological and medical sciences; Capsule Endoscopy; Cattle; Cell Line, Tumor; Cell Survival - drug effects; cytotoxicity; Digestion; enteral feeding; fasting; Feeding. Feeding behavior; Female; fluorescence emission spectroscopy; Fundamental and applied biological sciences. Psychology; gastric juice; Gastric Juice - enzymology; Gastric Juice - metabolism; Gastric Mucosa - enzymology; Gastric Mucosa - secretion; high performance liquid chromatography; Humans; image analysis; infrared spectroscopy; ingestion; Innovative Techniques; Intubation, Gastrointestinal; isoelectric point; lactalbumin; Lactalbumin - adverse effects; Lactalbumin - chemistry; Lactalbumin - metabolism; Lactalbumin - pharmacology; Male; oleic acid; Oleic Acid - chemistry; Oleic Acid - metabolism; Oleic Acid - pharmacology; Oleic Acids - pharmacology; pepsin; Peptide Fragments - chemistry; Peptide Fragments - metabolism; Peptide Fragments - pharmacology; Peristalsis; polyacrylamide gel electrophoresis; Protein Unfolding; proteolysis; stomach; Stomach - physiology; sucrose; Vertebrates: anatomy and physiology, studies on body, several organs or systems; volunteers; In vivo digestion; α-Lactalbumin; Capsule endoscopy; Human and bovine α-lactalbumin made lethal to tumour cells (HAMLET and BAMLET); Nasogastric tubes; Human; Stomach; Bovine; Nutrition; Digestive system; Ox; Digestion; In vivo; Vertebrata; Mammalia; Animal; Nasogastric; Artiodactyla; Ungulata; Tumor cell

In the present study, structural changes in the milk protein α-lactalbumin (α-LA) and its proteolysis were investigated for the potential formation of protein–fatty acid complexes during in vivo gastric digestion. Capsule endoscopy allowed visualisation of the digestion of the test drinks, with naso...