Direct microRNA Sequencing Using Nanopore-Induced Phase-Shift Sequencing

• Published in: iScience Vol. 23; no. 3; p. 100916
• Main Authors: Zhang, Jinyue, Yan, Shuanghong, Chang, Le, Guo, Weiming, Wang, Yuqin, Wang, Yu, Zhang, Panke, Chen, Hong-Yuan, Huang, Shuo
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 27.03.2020; Elsevier
• Subjects: Analytical Chemistry; Biotechnology; Molecular Biology; Nanotechnology; Biotechnology; Analytical Chemistry; Molecular Biology; Nanotechnology

MicroRNAs (miRNAs) are a class of short non-coding RNAs that function in RNA silencing and post-transcriptional gene regulation. However, direct characterization of miRNA is challenging due to its unique properties such as its low abundance, sequence similarities, and short length. Although urgently...