Structure and flexibility of the tropomyosin overlap junction

• Published in: Biochemical and biophysical research communications Vol. 446; no. 1; pp. 304 - 308
• Main Authors: Li, Xiaochuan Edward, Orzechowski, Marek, Lehman, William, Fischer, Stefan
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 28.03.2014
• Subjects: Actin; Actins - chemistry; Animals; Coiled-coil; Crystallography, X-Ray; Humans; Models, Molecular; Molecular Dynamics; Molecular Dynamics Simulation; Muscle regulation; Muscle, Skeletal - chemistry; Muscle, Smooth - chemistry; Nuclear Magnetic Resonance, Biomolecular; Protein Structure, Secondary; Protein Structure, Tertiary; Recombinant Fusion Proteins - chemistry; Thin filaments; Tropomyosin - chemistry; Thin filaments; Coiled-coil; Molecular Dynamics; Muscle regulation; Actin

•Head-to-tail linkage is required for tropomyosin cable formation on F-actin.•Molecular dynamics of tropomyosin overlap domains were carried out.•N- to C-terminal coiled-coils face each other at an orthogonal angle.•The bending angle of the overlap domain and the rest of the molecule are comparable....