High-speed AFM height spectroscopy reveals µs-dynamics of unlabeled biomolecules

Dynamics are fundamental to the functions of biomolecules and can occur on a wide range of time and length scales. Here we develop and apply high-speed AFM height spectroscopy (HS-AFM-HS), a technique whereby we monitor the sensing of a HS-AFM tip at a fixed position to directly detect the motions o...

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Published inNature communications Vol. 9; no. 1; pp. 4983 - 11
Main Authors Heath, George R., Scheuring, Simon
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 26.11.2018
Nature Publishing Group
Nature Portfolio
Subjects
147/3
631/1647/2204/1262
631/337/2265
631/57/2270
631/57/2271
639/766/747
Annexin A5
Biomolecules
Cell Membrane
Diffusion
High speed
Humanities and Social Sciences
Kinetics
Membranes
Microscopes
Microscopy, Atomic Force - methods
Models, Biological
multidisciplinary
Position sensing
Rotation
Science
Science (multidisciplinary)
Self-assembly
Spectroscopy
Spectrum Analysis
Staining and Labeling
Time Factors
Time-Lapse Imaging
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Abstract Dynamics are fundamental to the functions of biomolecules and can occur on a wide range of time and length scales. Here we develop and apply high-speed AFM height spectroscopy (HS-AFM-HS), a technique whereby we monitor the sensing of a HS-AFM tip at a fixed position to directly detect the motions of unlabeled molecules underneath. This gives Angstrom spatial and microsecond temporal resolutions. In conjunction with HS-AFM imaging modes to precisely locate areas of interest, HS-AFM-HS measures simultaneously surface concentrations, diffusion coefficients and oligomer sizes of annexin-V on model membranes to decipher key kinetics allowing us to describe the entire annexin-V membrane-association and self-assembly process in great detail and quantitatively. This work displays how HS-AFM-HS can assess the dynamics of unlabeled bio-molecules over several orders of magnitude and separate the various dynamic components spatiotemporally. The dynamics of biomolecules can occur over a wide range of time and length scales. Here the authors develop a high-speed AFM height spectroscopy method to directly detect the motion of unlabeled molecules at Angstrom spatial and microsecond temporal resolution.
AbstractList Dynamics are fundamental to the functions of biomolecules and can occur on a wide range of time and length scales. Here we develop and apply high-speed AFM height spectroscopy (HS-AFM-HS), a technique whereby we monitor the sensing of a HS-AFM tip at a fixed position to directly detect the motions of unlabeled molecules underneath. This gives Angstrom spatial and microsecond temporal resolutions. In conjunction with HS-AFM imaging modes to precisely locate areas of interest, HS-AFM-HS measures simultaneously surface concentrations, diffusion coefficients and oligomer sizes of annexin-V on model membranes to decipher key kinetics allowing us to describe the entire annexin-V membrane-association and self-assembly process in great detail and quantitatively. This work displays how HS-AFM-HS can assess the dynamics of unlabeled bio-molecules over several orders of magnitude and separate the various dynamic components spatiotemporally. The dynamics of biomolecules can occur over a wide range of time and length scales. Here the authors develop a high-speed AFM height spectroscopy method to directly detect the motion of unlabeled molecules at Angstrom spatial and microsecond temporal resolution.
Dynamics are fundamental to the functions of biomolecules and can occur on a wide range of time and length scales. Here we develop and apply high-speed AFM height spectroscopy (HS-AFM-HS), a technique whereby we monitor the sensing of a HS-AFM tip at a fixed position to directly detect the motions of unlabeled molecules underneath. This gives Angstrom spatial and microsecond temporal resolutions. In conjunction with HS-AFM imaging modes to precisely locate areas of interest, HS-AFM-HS measures simultaneously surface concentrations, diffusion coefficients and oligomer sizes of annexin-V on model membranes to decipher key kinetics allowing us to describe the entire annexin-V membrane-association and self-assembly process in great detail and quantitatively. This work displays how HS-AFM-HS can assess the dynamics of unlabeled bio-molecules over several orders of magnitude and separate the various dynamic components spatiotemporally.
The dynamics of biomolecules can occur over a wide range of time and length scales. Here the authors develop a high-speed AFM height spectroscopy method to directly detect the motion of unlabeled molecules at Angstrom spatial and microsecond temporal resolution.
Dynamics are fundamental to the functions of biomolecules and can occur on a wide range of time and length scales. Here we develop and apply high-speed AFM height spectroscopy (HS-AFM-HS), a technique whereby we monitor the sensing of a HS-AFM tip at a fixed position to directly detect the motions of unlabeled molecules underneath. This gives Angstrom spatial and microsecond temporal resolutions. In conjunction with HS-AFM imaging modes to precisely locate areas of interest, HS-AFM-HS measures simultaneously surface concentrations, diffusion coefficients and oligomer sizes of annexin-V on model membranes to decipher key kinetics allowing us to describe the entire annexin-V membrane-association and self-assembly process in great detail and quantitatively. This work displays how HS-AFM-HS can assess the dynamics of unlabeled bio-molecules over several orders of magnitude and separate the various dynamic components spatiotemporally.
ArticleNumber 4983
Author Heath, George R.
Scheuring, Simon
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SSID ssj0000391844
Score 2.5490727
Snippet Dynamics are fundamental to the functions of biomolecules and can occur on a wide range of time and length scales. Here we develop and apply high-speed AFM...
Dynamics are fundamental to the functions of biomolecules and can occur on a wide range of time and length scales. Here we develop and apply high-speed AFM...
The dynamics of biomolecules can occur over a wide range of time and length scales. Here the authors develop a high-speed AFM height spectroscopy method to...
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pubmedcentral
proquest
crossref
pubmed
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SourceType Open Website
Open Access Repository
Aggregation Database
Index Database
Publisher
StartPage 4983
SubjectTerms 147/3
631/1647/2204/1262
631/337/2265
631/57/2270
631/57/2271
639/766/747
Annexin A5
Biomolecules
Cell Membrane
Diffusion
High speed
Humanities and Social Sciences
Kinetics
Membranes
Microscopes
Microscopy, Atomic Force - methods
Models, Biological
multidisciplinary
Position sensing
Rotation
Science
Science (multidisciplinary)
Self-assembly
Spectroscopy
Spectrum Analysis
Staining and Labeling
Time Factors
Time-Lapse Imaging
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Title High-speed AFM height spectroscopy reveals µs-dynamics of unlabeled biomolecules
URI https://link.springer.com/article/10.1038/s41467-018-07512-3
https://www.ncbi.nlm.nih.gov/pubmed/30478320
https://www.proquest.com/docview/2138057389
https://search.proquest.com/docview/2138649306
https://pubmed.ncbi.nlm.nih.gov/PMC6255864
https://doaj.org/article/10b963554d2e41b2b5ae312445bcf43c
Volume 9
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