The functions of IL-23 and IL-2 on driving autoimmune effector T-helper 17 cells into the memory pool in dry eye disease

• Published in: Mucosal immunology Vol. 14; no. 1; pp. 177 - 186
• Main Authors: Chen, Yihe, Shao, Chunyi, Fan, Nai-Wen, Nakao, Takeshi, Amouzegar, Afsaneh, Chauhan, Sunil K., Dana, Reza
• Format: Journal Article
• Language: English
• Published: New York Elsevier Inc 01.01.2021; Nature Publishing Group US; Elsevier Limited
• Subjects: Allergology; Animal models; Animals; Antibodies; Apoptosis - genetics; Apoptosis - immunology; Autoimmune diseases; Autoimmune Diseases - etiology; Autoimmune Diseases - metabolism; Autoimmune Diseases - pathology; Autoimmunity; Biomarkers; Biomedical and Life Sciences; Biomedicine; Cell culture; Contraction; Disease Models, Animal; Disease Susceptibility; Dry Eye Syndromes - etiology; Dry Eye Syndromes - metabolism; Dry Eye Syndromes - pathology; Effector cells; Eye diseases; Gastroenterology; Helper cells; Humans; Immunologic Memory; Immunology; Inflammation; Interleukin 15 receptors; Interleukin 2; Interleukin 23; Interleukin-2 - genetics; Interleukin-2 - metabolism; Interleukin-23 - genetics; Interleukin-23 - metabolism; Lymphocytes T; Mice; Signal Transduction; Th17 Cells - immunology; Th17 Cells - metabolism
• DOI: 10.1038/s41385-020-0289-3
• ISSN: 1933-0219; 1935-3456

Long-lived memory T-helper 17 (Th17) cells actively mediate the chronic inflammation in autoimmune disorders, including dry eye disease (DED). The mechanisms responsible for the maintenance and reactivation of these cells in autoimmunity have been subject of investigation. However, the process through which memory Th17 are generated from their effector precursors remains to be elucidated. Herein, using our murine model of DED, we detect a linear transition from effector-to-memory Th17 cells during the abatement phase of acute inflammation, which is accompanied by persistently high levels of IL-23 and diminished levels of IL-2. In addition, in vitro culture of effector Th17 cells derived from the DED animals with IL-23, but not IL-2, leads to significant generation of memory Th17 cells, along with upregulated expression levels of IL-7R and IL-15R by these cells. Furthermore, supplementation of IL-2 abolishes and blockade of IL-2 enhances IL-23-induced generation of memory Th17 cells in vitro. Finally, in vivo blockade of IL-23 signaling during the contraction phase of primary response inhibits the generation of memory Th17 cells from their effector precursors. Together, our data demonstrate a new dichotomy between IL-23 and IL-2 in driving effector Th17 cells into the memory pool in autoimmune-mediated ocular surface inflammation.