Large-scale reduction of the Bacillus subtilis genome: consequences for the transcriptional network, resource allocation, and metabolism

• Published in: Genome research Vol. 27; no. 2; pp. 289 - 299
• Main Authors: Reuß, Daniel R., Altenbuchner, Josef, Mäder, Ulrike, Rath, Hermann, Ischebeck, Till, Sappa, Praveen Kumar, Thürmer, Andrea, Guérin, Cyprien, Nicolas, Pierre, Steil, Leif, Zhu, Bingyao, Feussner, Ivo, Klumpp, Stefan, Daniel, Rolf, Commichau, Fabian M., Völker, Uwe, Stülke, Jörg
• Format: Journal Article
• Language: English
• Published: United States Cold Spring Harbor Laboratory Press 01.02.2017
• Subjects: Bacillus subtilis; Bacillus subtilis - genetics; Bacillus subtilis - metabolism; Bacterial Proteins - genetics; Computer Science; Gene Expression Regulation, Bacterial - genetics; Gene Regulatory Networks - genetics; Genes, Essential - genetics; Genome, Bacterial - genetics; Life Sciences; Mathematics; Transcription, Genetic; eschrichia-coli; glutamate biosynthesis; expression; cells; essential genes

Understanding cellular life requires a comprehensive knowledge of the essential cellular functions, the components involved, and their interactions. Minimized genomes are an important tool to gain this knowledge. We have constructed strains of the model bacterium, Bacillus subtilis , whose genomes h...