Muscarinic M1 and M2 receptor subtypes play opposite roles in LPS-induced septic shock

• Published in: Pharmacological reports Vol. 71; no. 6; pp. 1108 - 1114
• Main Authors: Wang, Zhen, Li, Mingyi, Liu, Lu, Geng, Bin
• Format: Journal Article
• Language: English
• Published: Cham Elsevier B.V 01.12.2019; Springer International Publishing
• Subjects: Animals; Atropine - pharmacology; Cholinergic anti-inflammatory pathway; Cytokines - metabolism; Drug Safety and Pharmacovigilance; Inflammation; Lipopolysaccharides; Liver - pathology; Lung - pathology; Macrophages - metabolism; Male; Mice; Mice, Inbred C57BL; Muscarinic cholinergic receptor; Neutrophil Infiltration - drug effects; Original Article; Pharmacotherapy; Pharmacy; Pirenzepine - analogs & derivatives; Pirenzepine - pharmacology; Receptor, Muscarinic M1 - antagonists & inhibitors; Receptor, Muscarinic M1 - physiology; Receptor, Muscarinic M2 - antagonists & inhibitors; Receptor, Muscarinic M2 - physiology; Sepsis; Shock, Septic - chemically induced; Shock, Septic - drug therapy; Shock, Septic - mortality; Shock, Septic - physiopathology; Tumor Necrosis Factor-alpha - blood; Tumor Necrosis Factor-alpha - metabolism; Muscarinic cholinergic receptor; Sepsis; Inflammation; Cholinergic anti-inflammatory pathway

To compare pharmacologic effects of pirenzepine and AF-DX116, a selective competitive antagonist for M1 and M2 subtype muscarinic cholinergic receptors (mAChRs), respectively, with atropine, a non-selective competitive antagonist for mAChRs, on Lipopolysaccharide (LPS). Male C57BL/6 mice were used t...