Extraction of nuclei from archived postmortem tissues for single-nucleus sequencing applications

• Published in: Nature protocols Vol. 16; no. 6; pp. 2788 - 2801
• Main Authors: Maitra, Malosree, Nagy, Corina, Chawla, Anjali, Wang, Yu Chang, Nascimento, Camila, Suderman, Matthew, Théroux, Jean-François, Mechawar, Naguib, Ragoussis, Jiannis, Turecki, Gustavo
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.06.2021; Nature Publishing Group
• Subjects: 631/1647/2017; 631/1647/2210/2211; 631/208/514; 631/378; Analytical Chemistry; Biological Techniques; Biomedical and Life Sciences; Cell Nucleus; Chromatin; Chromium; Computational Biology/Bioinformatics; Deoxyribonucleic acid; DNA; DNA sequencing; Fluorescence; Humans; Laboratories; Life Sciences; Medical jurisprudence; Medical research; Medicine, Experimental; Methods; Microarrays; Nuclei; Nuclei (cytology); Nucleic acids; Nucleotide sequencing; Organic Chemistry; Protocol; Ribonucleic acid; RNA; Separation; Sequence Analysis, DNA; Single-Cell Analysis - methods; snRNA; Tissues; Transposase; Ultracentrifugation; Workflow; Canada

Single-cell and single-nucleus sequencing techniques are a burgeoning field with various biological, biomedical and clinical applications. Numerous high- and low-throughput methods have been developed for sequencing the RNA and DNA content of single cells. However, for all these methods, the key req...