Human Keratinocytes Synthesize, Secrete, and Deposit Fibronectin in the Pericellular Matrix

• Published in: Journal of investigative dermatology Vol. 82; no. 6; pp. 580 - 586
• Main Authors: Kubo, Miyoko, Norris, David A., Howell, Samuel E., Ryan, Sheli R, Clark, Richard A F
• Format: Journal Article
• Language: English
• Published: Danvers, MA Elsevier Inc 01.06.1984; Nature Publishing
• Subjects: Biological and medical sciences; Enzyme-Linked Immunosorbent Assay; Epidermal Cells; Epidermis - metabolism; Fibronectins - biosynthesis; Fibronectins - metabolism; Fluorescent Antibody Technique; Fundamental and applied biological sciences. Psychology; Humans; Vertebrates: skin, associated glands, phaneres, light organs, various exocrine glands (salt gland, uropygial gland...), adipose tissue, connective tissue; Human; Epidermis; Keratinocyte; Extracellular matrix; Biosynthesis; Skin; Metabolism; Fibronectin

Fibronectin production by human keratinocytes cultured in serum-free, low-calcium medium without a fibroblast feeder layer was examined using several techniques. Immunohistochemical examination confirmed that the cultures were not contaminated with fibroblasts or Langerhans cells. By indirect immuno...