Novel method for the quantification of inorganic polyphosphate (iPoP) in Saccharomyces cerevisiae shows dependence of iPoP content on the growth phase

• Published in: Archives of microbiology Vol. 184; no. 2; pp. 129 - 136
• Main Authors: Werner, Thomas P, Amrhein, Nikolaus, Freimoser, Florian M
• Format: Journal Article
• Language: English
• Published: Heidelberg Berlin/Heidelberg : Springer-Verlag 01.11.2005; Berlin Springer; Springer Nature B.V
• Subjects: Bacteriology; Biological and medical sciences; Culture Media; Diauxic shift; Fundamental and applied biological sciences. Psychology; Glucose - metabolism; Growth conditions; Growth, nutrition, metabolism, transports, enzymes. Molecular biology; Inorganic polyphosphate; Microbiology; Mycology; Phosphates; Phosphates - metabolism; PolyP; Polyphosphate quantification method; Polyphosphates - analysis; Polyphosphates - metabolism; Saccharomyces cerevisiae; Saccharomyces cerevisiae - chemistry; Saccharomyces cerevisiae - growth & development; Saccharomyces cerevisiae - metabolism; Yeast; Yeasts; “Overplus,” Growth phase; Diauxic shift; Inorganic polyphosphate; Yeast; Overplus, Growth phase; Enzyme; Quantization; Exopolyphosphatase; Minerals; Polyphosphates; Fungi; Hydrolases; Ascomycetes; Saccharomyces cerevisiae; Thallophyta; PolyP; Polyphosphate quantification method

Inorganic polyphosphate (iPoP)--linear chains of up to hundreds of phosphate residues--is ubiquitous in nature and appears to be involved in many different cellular processes. In Saccharomyces cerevisiae, iPoP has been detected in high concentrations, especially after transfer of phosphate-deprived...