Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM

• Published in: Nature methods Vol. 10; no. 6; pp. 584 - 590
• Main Authors: Li, Xueming, Mooney, Paul, Zheng, Shawn, Booth, Christopher R, Braunfeld, Michael B, Gubbens, Sander, Agard, David A, Cheng, Yifan
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.06.2013; Nature Publishing Group
• Subjects: 631/114/1314; 631/1647/2258/1258/1259; 631/1647/328/1259; 631/45/612; Bioinformatics; Biological Microscopy; Biological Techniques; Biomedical Engineering/Biotechnology; Cryoelectron microscopy; Cryoelectron Microscopy - methods; Data acquisition; Electrons; Imaging, Three-Dimensional - methods; Life Sciences; Methods; Microscopy; Motion; Proteasome Endopeptidase Complex - ultrastructure; Proteins; Proteomics; Thermoplasma - enzymology; Three-dimensional display systems; United States

The combination of a direct electron-detection camera that can count individual electrons and an algorithm for correcting for beam-induced motion in cryo-EM will facilitate determination of three-dimensional structures of smaller, lower-symmetry macromolecular complexes to higher resolution than pre...