Isolation and Characterization of a Single-Stranded RNA Virus Infecting the Bloom-Forming Diatom Chaetoceros socialis

• Published in: Applied and Environmental Microbiology Vol. 75; no. 8; pp. 2375 - 2381
• Main Authors: Tomaru, Yuji, Takao, Yoshitake, Suzuki, Hidekazu, Nagumo, Tamotsu, Nagasaki, Keizo
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.04.2009; American Society for Microbiology (ASM)
• Subjects: Algae; Amino acid sequence; Biological and medical sciences; Cells; Chaetoceros; Cluster Analysis; Diatoms - virology; Fundamental and applied biological sciences. Psychology; Japan; Microbial Ecology; Microbiology; Microscopy, Electron, Transmission; Molecular Sequence Data; Molecular Weight; Open Reading Frames; Phylogeny; RNA polymerase; RNA Viruses - classification; RNA Viruses - genetics; RNA Viruses - isolation & purification; RNA Viruses - ultrastructure; RNA, Viral - genetics; Seawater - virology; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Survival analysis; Viral Proteins - chemistry; Viral Proteins - genetics; Virion - ultrastructure; Viruses; Viruses, Unclassified - classification; Viruses, Unclassified - genetics; Viruses, Unclassified - isolation & purification; Viruses, Unclassified - ultrastructure; Japan; Hiroshima Bay; Characterization; Heterokontophyta; Bacillariophyta; RNA virus; Algae; Bloom; Isolation
• ISSN: 0099-2240; 1098-5336; 1098-6596
• DOI: 10.1128/AEM.02580-08

Diatoms are very significant primary producers in the world's oceans. Various environmental factors affect the depletion of diatom populations. The importance of viruses as a potential mortality source has recently been recognized. We isolated and characterized a new diatom virus (Chaetoceros socialis f. radians RNA virus [CsfrRNAV]) causing the lysis of the bloom-forming species Chaetoceros socialis Lauder f. radians (Schütt) Proschkina-Lavrenko. The virus infectious to C. socialis f. radians was isolated from water samples collected in Hiroshima Bay. Here we show the physiology, morphology, and genome characteristics of the virus clone. Virions were 22 nm in diameter and accumulated in the cytoplasm of the host cells. The latent period and the burst size were estimated to be <48 h and 66 infectious units per host cell, respectively. CsfrRNAV harbors a single-stranded RNA (ssRNA) genome and encodes at least three polypeptides of 32.0, 28.5, and 25.0 kDa. Sequencing analysis shows the length of the genome is 9,467 bases, excluding a poly(A) tail. The monophyly of CsfrRNAV and other diatom-infecting RNA viruses, Rhizosolenia setigera RNA virus and Chaetoceros tenuissimus RNA virus, was strongly supported by phylogenetic analysis based on the amino acid sequence of the RNA-dependent RNA polymerase domains. This suggested a new ssRNA virus family, Bacillariornaviridae. This discovery of CsfrRNAV may aid in further understanding the ecological dynamics of the C. socialis f. radians population in nature and the relationships between ssRNA diatom viruses and their hosts.