Recombinant Human IgA Expressed in Insect Cells

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 91; no. 18; pp. 8348 - 8352
• Main Authors: Carayannopoulos, Leonidas, Max, Edward E., Capra, J. Donald
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 30.08.1994; National Acad Sciences; National Academy of Sciences
• Subjects: Animals; Antibodies; Baculoviridae - genetics; Biochemistry; Complement C3 - metabolism; Erythrocytes; Experimentation; Genes; Genes, Immunoglobulin; Genetic Vectors; Glycosylation; Humans; Immunity (Disease); Immunoglobulin A - genetics; Immunoglobulin A - metabolism; Immunoglobulin alpha-Chains - genetics; Immunoglobulin J-Chains - genetics; Immunoglobulin kappa-Chains - genetics; Immunology; Insect viruses; Insecta; Insects; Mice; Molecules; Moths; Oligosaccharides; Protein Processing, Post-Translational; Proteins; Receptors; Receptors, Fc - metabolism; Recombinant Fusion Proteins - immunology; Recombinant Fusion Proteins - metabolism; Viruses

IgA serves as the first line of humoral defense at all mucosal surfaces and is present in large quantities in serum. To map the sites of interaction of immune effector molecules with the IgA constant region (Cα), we have expressed soluble, chimeric human IgA in insect cells using recombinant baculov...