Establishment of sandwich ELISA for soluble alpha-Klotho measurement: Age-dependent change of soluble alpha-Klotho levels in healthy subjects

• Published in: Biochemical and biophysical research communications Vol. 398; no. 3; pp. 513 - 518
• Main Authors: Yamazaki, Yuji, Imura, Akihiro, Urakawa, Itaru, Shimada, Takashi, Murakami, Junko, Aono, Yukiko, Hasegawa, Hisashi, Yamashita, Takeyoshi, Nakatani, Kimihiko, Saito, Yoshihiko, Okamoto, Nozomi, Kurumatani, Norio, Namba, Noriyuki, Kitaoka, Taichi, Ozono, Keiichi, Sakai, Tomoyuki, Hataya, Hiroshi, Ichikawa, Shoji, Imel, Erik A., Econs, Michael J., Nabeshima, Yo-ichi
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.07.2010
• Subjects: Adult; Age Factors; Aged; Aged, 80 and over; Aging - blood; Alpha-Klotho; Animals; Antibodies, Monoclonal - immunology; Calcium; Calcium - blood; Child; ELISA; Enzyme-Linked Immunosorbent Assay; Female; Glucuronidase - blood; Glucuronidase - genetics; Glucuronidase - immunology; Humans; Male; Mice; Middle Aged; Mutation; Phosphate; Tumoral calcinosis; Tumoral calcinosis; Calcium; Alpha-Klotho; Phosphate; ELISA

► We established a detection system to measure human serum soluble α-Klotho (sαKl). ► We found that Age influences serum sαKl levels in a normal population. ► This detection system should be an excellent tool for investigating sαKl functions. α-Klotho (αKl) regulates mineral metabolism such as calci...