short peptide from frog skin accelerates diabetic wound healing

• Published in: The FEBS journal Vol. 281; no. 20; pp. 4633 - 4643
• Main Authors: Liu, Han, Duan, Zilei, Tang, Jing, Lv, Qiumin, Rong, Mingqiang, Lai, Ren
• Format: Journal Article
• Language: English
• Published: England Blackwell 01.10.2014; Blackwell Publishing Ltd
• Subjects: amino acid sequences; amphibian; Angiogenesis; angiogenic proteins; animal models; Animals; anti-inflammatory activity; anti‐inflammatory; Anura; Blotting, Western; Diabetes; Diabetes Mellitus, Experimental - drug therapy; Diabetes Mellitus, Experimental - physiopathology; diabetic wounds; Disease Models, Animal; drugs; Enzyme-Linked Immunosorbent Assay; Frogs; healing; Immunoenzyme Techniques; inflammation; Male; Mice; Mice, Inbred BALB C; Mice, Obese; Neovascularization, Physiologic - drug effects; Odorrana; Peptide Fragments - pharmacology; Peptides; Pharmacology; Skin - blood supply; Skin - injuries; Skin - metabolism; tissue repair; Wound Healing; anti-inflammatory; wound healing; amphibian; angiogenesis; diabetic wounds
• ISSN: 1742-464X; 1742-4658
• DOI: 10.1111/febs.12968

Delayed wound healing will result in the development of chronic wounds in some diseases, such as diabetes. Amphibian skins possess excellent wound‐healing ability and represent a resource for prospective wound‐healing promoting compounds. A potential wound‐healing promoting peptide (CW49; amino acid sequence APFRMGICTTN) was identified from the frog skin of Odorrana grahami. It promotes wound healing in a murine model with a full‐thickness dermal wound in both normal and diabetic animals. In addition to its strong angiogenic ability with respect to the upregulation of some angiogenic proteins, CW49 also showed a significant anti‐inflammatory effect in diabetic wounds, which was very important for healing chronic wounds. CW49 had little effect on re‐epithelialization, resulting in no significant effect on wound closure rate compared to a vehicle control. Altogether, this indicated that CW49 might accelerate diabetic wound healing by promoting angiogenesis and preventing any excessive inflammatory response. Considering its favorable traits as a small peptide that significantly promotes angiogenesis, CW49 might be an excellent candidate or template for the development of a drug for use in the treatment of diabetic wounds.