Alteration of cell membrane proteoglycans impairs FSH receptor/Gs coupling and ERK activation through PP2A-dependent mechanisms in immature rat Sertoli cells

• Published in: Biochimica et biophysica acta Vol. 1830; no. 6; pp. 3466 - 3475
• Main Authors: Levallet, Guénaëlle, Bonnamy, Pierre-Jacques, Levallet, Jérôme
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.06.2013; Elsevier
• Subjects: Animals; cAMP-dependent protein kinase; cell membranes; cell proliferation; Cells, Cultured; cholera; cyclic AMP; Cyclic AMP - metabolism; Cyclic AMP-Dependent Protein Kinases - metabolism; follicle-stimulating hormone; follicle-stimulating hormone receptors; FSH signaling; Heparan sulfate proteoglycan; Life Sciences; Male; MAP Kinase Signaling System - physiology; mitogen-activated protein kinase; Mitogen-Activated Protein Kinase 3 - metabolism; okadaic acid; phosphorylation; Protein Phosphatase 2 - metabolism; Protein phosphatase 2A; proteoglycans; Rats; Rats, Sprague-Dawley; Receptors, FSH - agonists; Receptors, FSH - metabolism; Second Messenger Systems - physiology; Sertoli cell; Sertoli cells; Sertoli Cells - metabolism; Sheep; sodium chlorate; Syndecan-1; Syndecan-1 - metabolism; Sertoli cell; HSPG; PP2A; MAPK; SC; OA; FSH signaling; FBS; Syndecan-1; PGs; ERK; Heparan sulfate proteoglycan; Protein phosphatase 2A; INTACT-CELLS; EPITHELIAL-CELLS; AGE-RELATED-CHANGES; BETA-ARRESTINS; BINDING PROTEINS; FOLLICLE-STIMULATING-HORMONE; HEPARAN-SULFATE PROTEOGLYCANS; SIGNAL-REGULATED KINASE; ESTRADIOL PRODUCTION

During the pre-pubertal life, the cessation of Sertoli cell proliferation and the onset of differentiation are associated with a shift in the FSH-mediated signaling leading to inhibition of the ERK-mitogenic pathway and to a concomitant sensitization of cAMP/PKA pathway. To highlight the role of cel...