Direct observation of structure-function relationship in a nucleic acid-processing enzyme

• Published in: Science (American Association for the Advancement of Science) Vol. 348; no. 6232; pp. 352 - 354
• Main Authors: Comstock, Matthew J., Whitley, Kevin D., Jia, Haifeng, Sokoloski, Joshua, Lohman, Timothy M., Ha, Taekjip, Chemla, Yann R.
• Format: Journal Article
• Language: English
• Published: Washington American Association for the Advancement of Science 17.04.2015; The American Association for the Advancement of Science
• Subjects: Bacteria; Crosslinking; Deoxyribonucleic acid; DNA; DNA repair; E coli; Enzymes; Escherichia coli; Fluorescence; Fluorescence microscopy; Microscopy; Nucleic acids; Protein expression; Proteins; Winding

The relationship between protein three-dimensional structure and function is essential for mechanism determination. Unfortunately, most techniques do not provide a direct measurement of this relationship. Structural data are typically limited to static pictures, and function must be inferred. Conver...