Mixed and membrane-separated culturing of synthetic cyanobacteria-yeast consortia reveals metabolic cross-talk mimicking natural cyanolichens
Metabolite exchange mediates crucial interactions in microbial communities, significantly impacting global carbon and nitrogen cycling. Understanding these chemically-mediated interactions is essential for elucidating natural community functions and developing engineered synthetic communities. This...
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| Published in | Scientific reports Vol. 14; no. 1; pp. 25303 - 19 |
|---|---|
| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | English |
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London
Nature Publishing Group UK
25.10.2024
Nature Publishing Group Nature Portfolio |
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| Abstract | Metabolite exchange mediates crucial interactions in microbial communities, significantly impacting global carbon and nitrogen cycling. Understanding these chemically-mediated interactions is essential for elucidating natural community functions and developing engineered synthetic communities. This study investigated membrane-separated bioreactors (mBRs) as a novel tool to identify transient metabolites and their producers/consumers in mixed microbial communities. We compared three co-culture methods (direct mixed, 2-chamber mBR, and 3-chamber mBR) to grow a synthetic binary community of the cyanobacterium
Synechococcus elongatus
PCC 7942 and the fungus
Rhodotorula toruloides
NBRC 0880, as well as axenic
S. elongatus
. Despite not being natural lichen constituents, these organisms exhibited interactions resembling those in cyanolichens.
S. elongatus
fixed CO
2
into sugars as the primary shared metabolite, while
R. toruloides
secreted various biochemicals, predominantly sugar alcohols, mirroring the metabolite exchange observed in natural lichens. The mBR systems successfully captured metabolite gradients and revealed rapidly consumed compounds, including TCA cycle intermediates and amino acids. Our approach demonstrated that the 2-chamber mBR optimally balanced metabolite exchange and growth dynamics. This study provides insights into cross-species metabolic interactions and presents a valuable tool for investigating and engineering synthetic microbial communities with potential applications in biotechnology and environmental science. |
|---|---|
| AbstractList | Metabolite exchange mediates crucial interactions in microbial communities, significantly impacting global carbon and nitrogen cycling. Understanding these chemically-mediated interactions is essential for elucidating natural community functions and developing engineered synthetic communities. This study investigated membrane-separated bioreactors (mBRs) as a novel tool to identify transient metabolites and their producers/consumers in mixed microbial communities. We compared three co-culture methods (direct mixed, 2-chamber mBR, and 3-chamber mBR) to grow a synthetic binary community of the cyanobacterium
Synechococcus elongatus
PCC 7942 and the fungus
Rhodotorula toruloides
NBRC 0880, as well as axenic
S. elongatus
. Despite not being natural lichen constituents, these organisms exhibited interactions resembling those in cyanolichens.
S. elongatus
fixed CO
2
into sugars as the primary shared metabolite, while
R. toruloides
secreted various biochemicals, predominantly sugar alcohols, mirroring the metabolite exchange observed in natural lichens. The mBR systems successfully captured metabolite gradients and revealed rapidly consumed compounds, including TCA cycle intermediates and amino acids. Our approach demonstrated that the 2-chamber mBR optimally balanced metabolite exchange and growth dynamics. This study provides insights into cross-species metabolic interactions and presents a valuable tool for investigating and engineering synthetic microbial communities with potential applications in biotechnology and environmental science. Abstract Metabolite exchange mediates crucial interactions in microbial communities, significantly impacting global carbon and nitrogen cycling. Understanding these chemically-mediated interactions is essential for elucidating natural community functions and developing engineered synthetic communities. This study investigated membrane-separated bioreactors (mBRs) as a novel tool to identify transient metabolites and their producers/consumers in mixed microbial communities. We compared three co-culture methods (direct mixed, 2-chamber mBR, and 3-chamber mBR) to grow a synthetic binary community of the cyanobacterium Synechococcus elongatus PCC 7942 and the fungus Rhodotorula toruloides NBRC 0880, as well as axenic S. elongatus. Despite not being natural lichen constituents, these organisms exhibited interactions resembling those in cyanolichens. S. elongatus fixed CO2 into sugars as the primary shared metabolite, while R. toruloides secreted various biochemicals, predominantly sugar alcohols, mirroring the metabolite exchange observed in natural lichens. The mBR systems successfully captured metabolite gradients and revealed rapidly consumed compounds, including TCA cycle intermediates and amino acids. Our approach demonstrated that the 2-chamber mBR optimally balanced metabolite exchange and growth dynamics. This study provides insights into cross-species metabolic interactions and presents a valuable tool for investigating and engineering synthetic microbial communities with potential applications in biotechnology and environmental science. Metabolite exchange mediates crucial interactions in microbial communities, significantly impacting global carbon and nitrogen cycling. Understanding these chemically-mediated interactions is essential for elucidating natural community functions and developing engineered synthetic communities. This study investigated membrane-separated bioreactors (mBRs) as a novel tool to identify transient metabolites and their producers/consumers in mixed microbial communities. We compared three co-culture methods (direct mixed, 2-chamber mBR, and 3-chamber mBR) to grow a synthetic binary community of the cyanobacterium Synechococcus elongatus PCC 7942 and the fungus Rhodotorula toruloides NBRC 0880, as well as axenic S. elongatus. Despite not being natural lichen constituents, these organisms exhibited interactions resembling those in cyanolichens. S. elongatus fixed CO2 into sugars as the primary shared metabolite, while R. toruloides secreted various biochemicals, predominantly sugar alcohols, mirroring the metabolite exchange observed in natural lichens. The mBR systems successfully captured metabolite gradients and revealed rapidly consumed compounds, including TCA cycle intermediates and amino acids. Our approach demonstrated that the 2-chamber mBR optimally balanced metabolite exchange and growth dynamics. This study provides insights into cross-species metabolic interactions and presents a valuable tool for investigating and engineering synthetic microbial communities with potential applications in biotechnology and environmental science.Metabolite exchange mediates crucial interactions in microbial communities, significantly impacting global carbon and nitrogen cycling. Understanding these chemically-mediated interactions is essential for elucidating natural community functions and developing engineered synthetic communities. This study investigated membrane-separated bioreactors (mBRs) as a novel tool to identify transient metabolites and their producers/consumers in mixed microbial communities. We compared three co-culture methods (direct mixed, 2-chamber mBR, and 3-chamber mBR) to grow a synthetic binary community of the cyanobacterium Synechococcus elongatus PCC 7942 and the fungus Rhodotorula toruloides NBRC 0880, as well as axenic S. elongatus. Despite not being natural lichen constituents, these organisms exhibited interactions resembling those in cyanolichens. S. elongatus fixed CO2 into sugars as the primary shared metabolite, while R. toruloides secreted various biochemicals, predominantly sugar alcohols, mirroring the metabolite exchange observed in natural lichens. The mBR systems successfully captured metabolite gradients and revealed rapidly consumed compounds, including TCA cycle intermediates and amino acids. Our approach demonstrated that the 2-chamber mBR optimally balanced metabolite exchange and growth dynamics. This study provides insights into cross-species metabolic interactions and presents a valuable tool for investigating and engineering synthetic microbial communities with potential applications in biotechnology and environmental science. Metabolite exchange mediates crucial interactions in microbial communities, significantly impacting global carbon and nitrogen cycling. Understanding these chemically-mediated interactions is essential for elucidating natural community functions and developing engineered synthetic communities. This study investigated membrane-separated bioreactors (mBRs) as a novel tool to identify transient metabolites and their producers/consumers in mixed microbial communities. We compared three co-culture methods (direct mixed, 2-chamber mBR, and 3-chamber mBR) to grow a synthetic binary community of the cyanobacterium Synechococcus elongatus PCC 7942 and the fungus Rhodotorula toruloides NBRC 0880, as well as axenic S. elongatus. Despite not being natural lichen constituents, these organisms exhibited interactions resembling those in cyanolichens. S. elongatus fixed CO into sugars as the primary shared metabolite, while R. toruloides secreted various biochemicals, predominantly sugar alcohols, mirroring the metabolite exchange observed in natural lichens. The mBR systems successfully captured metabolite gradients and revealed rapidly consumed compounds, including TCA cycle intermediates and amino acids. Our approach demonstrated that the 2-chamber mBR optimally balanced metabolite exchange and growth dynamics. This study provides insights into cross-species metabolic interactions and presents a valuable tool for investigating and engineering synthetic microbial communities with potential applications in biotechnology and environmental science. Metabolite exchange mediates crucial interactions in microbial communities, significantly impacting global carbon and nitrogen cycling. Understanding these chemically-mediated interactions is essential for elucidating natural community functions and developing engineered synthetic communities. This study investigated membrane-separated bioreactors (mBRs) as a novel tool to identify transient metabolites and their producers/consumers in mixed microbial communities. We compared three co-culture methods (direct mixed, 2-chamber mBR, and 3-chamber mBR) to grow a synthetic binary community of the cyanobacterium Synechococcus elongatus PCC 7942 and the fungus Rhodotorula toruloides NBRC 0880, as well as axenic S. elongatus. Despite not being natural lichen constituents, these organisms exhibited interactions resembling those in cyanolichens. S. elongatus fixed CO2 into sugars as the primary shared metabolite, while R. toruloides secreted various biochemicals, predominantly sugar alcohols, mirroring the metabolite exchange observed in natural lichens. The mBR systems successfully captured metabolite gradients and revealed rapidly consumed compounds, including TCA cycle intermediates and amino acids. Our approach demonstrated that the 2-chamber mBR optimally balanced metabolite exchange and growth dynamics. This study provides insights into cross-species metabolic interactions and presents a valuable tool for investigating and engineering synthetic microbial communities with potential applications in biotechnology and environmental science. |
| ArticleNumber | 25303 |
| Author | Magnuson, Jon K Kim, Young-Mo Betenbaugh, Michael J Pomraning, Kyle R. Jenkins, Jackson P Bohutskyi, Pavlo Poirier, Brenton C |
| Author_xml | – sequence: 1 givenname: Pavlo surname: Bohutskyi fullname: Bohutskyi, Pavlo email: pavlo.bohutskyi@pnnl.gov organization: Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Department of Biological Systems Engineering, Washington State University – sequence: 2 givenname: Kyle R. surname: Pomraning fullname: Pomraning, Kyle R. organization: Energy and Environment Directorate, Pacific Northwest National Laboratory – sequence: 3 givenname: Jackson P surname: Jenkins fullname: Jenkins, Jackson P organization: Department of Chemical and Biomolecular Engineering, Johns Hopkins University – sequence: 4 givenname: Young-Mo surname: Kim fullname: Kim, Young-Mo organization: Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory – sequence: 5 givenname: Brenton C surname: Poirier fullname: Poirier, Brenton C organization: Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory – sequence: 6 givenname: Michael J surname: Betenbaugh fullname: Betenbaugh, Michael J organization: Department of Chemical and Biomolecular Engineering, Johns Hopkins University – sequence: 7 givenname: Jon K surname: Magnuson fullname: Magnuson, Jon K organization: Energy and Environment Directorate, Pacific Northwest National Laboratory |
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| Keywords | Membrane-separated bioreactor Synthetic lichen Metabolite exchange Phototroph-heterotroph co-culture Cross-feeding Microbial community |
| Language | English |
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| Snippet | Metabolite exchange mediates crucial interactions in microbial communities, significantly impacting global carbon and nitrogen cycling. Understanding these... Abstract Metabolite exchange mediates crucial interactions in microbial communities, significantly impacting global carbon and nitrogen cycling. Understanding... |
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| SubjectTerms | 631/326/2565 631/326/2565/855 Alcohols Amino acids BASIC BIOLOGICAL SCIENCES Bioreactors Bioreactors - microbiology Biotechnology Carbon cycle Carbon dioxide Coculture Techniques - methods Cross-feeding Cyanobacteria - genetics Cyanobacteria - metabolism Environmental science Germfree Humanities and Social Sciences Intermediates Lichens Lichens - metabolism Lichens - microbiology Membrane-separated bioreactor Metabolic engineering Metabolism Metabolite exchange Metabolites Microbial activity Microbial communities Microbial community Microbial Consortia Microbial ecology Microbial Interactions Microbiomes multidisciplinary Nitrogen cycle Phototroph-heterotroph co-culture Rhodotorula - genetics Rhodotorula - metabolism Science Science (multidisciplinary) Sugar Synechococcus - genetics Synechococcus - metabolism Synthetic lichen Tricarboxylic acid cycle Yeast |
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| Title | Mixed and membrane-separated culturing of synthetic cyanobacteria-yeast consortia reveals metabolic cross-talk mimicking natural cyanolichens |
| URI | https://link.springer.com/article/10.1038/s41598-024-74743-4 https://www.ncbi.nlm.nih.gov/pubmed/39455633 https://www.proquest.com/docview/3120699041 https://www.proquest.com/docview/3121058989 https://www.osti.gov/servlets/purl/2476534 https://pubmed.ncbi.nlm.nih.gov/PMC11511929 https://doaj.org/article/fa6d32f32f6b49aa84962ddd9048fc5a |
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