MicroRNA-15b Targets VEGF and Inhibits Angiogenesis in Proliferative Diabetic Retinopathy
Abstract Background Vascular endothelial growth factor (VEGF)-induced angiogenesis is a critical compensatory response to microvascular rarefaction in the diabetic retina that contributes to proliferative diabetic retinopathy (PDR). In this study, we sought to determine the role of specific micro ri...
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Published in | The journal of clinical endocrinology and metabolism Vol. 105; no. 11; pp. 1 - 3415 |
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Main Authors | , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
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Oxford University Press
01.11.2020
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Abstract | Abstract
Background
Vascular endothelial growth factor (VEGF)-induced angiogenesis is a critical compensatory response to microvascular rarefaction in the diabetic retina that contributes to proliferative diabetic retinopathy (PDR). In this study, we sought to determine the role of specific micro ribonucleic acids (RNAs) (miRs) associated with VEGF in patients with PDR pathology.
Methods
RNA sequencing was employed to detect differentially circulating miR associated with VEGF in patients with diabetes mellitus (DM), nonproliferative diabetic retinopathy (NPDR) and PDR. Quantitative real-time polymerase chain reaction was performed to measure the concentration of miR-15b in the serum of patients with DM (n = 115), NPDR (n = 47), or PDR (n = 76). The effects of miR-15b on DR and regulation of VEGF and endothelial cell function were also characterized.
Results
We demonstrated that circulating miR-15b was directly associated with VEGF compared with other miRs in patients with PDR. We found a significant inverse relationship between low levels of miR-15b and high levels of VEGF in patients with PDR when compared with the DM or NPDR groups. We found that miR-15b regulates the expression of VEGF by targeting the 3'-untranslated regions to inhibit its transcription. Similarly, overexpression of miR-15b suppressed vascular abnormalities in vivo in diabetic GK rats, inhibiting endothelial tube formation and VEGF expression.
Conclusion
Circulating miR-15b is associated with PDR and may be targeted to regulate VEGF expression and angiogenesis. |
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AbstractList | Background Vascular endothelial growth factor (VEGF)-induced angiogenesis is a critical compensatory response to microvascular rarefaction in the diabetic retina that contributes to proliferative diabetic retinopathy (PDR). In this study, we sought to determine the role of specific micro ribonucleic acids (RNAs) (miRs) associated with VEGF in patients with PDR pathology. Methods RNA sequencing was employed to detect differentially circulating miR associated with VEGF in patients with diabetes mellitus (DM), nonproliferative diabetic retinopathy (NPDR) and PDR. Quantitative real-time polymerase chain reaction was performed to measure the concentration of miR-15b in the serum of patients with DM (n = 115), NPDR (n = 47), or PDR (n = 76). The effects of miR-15b on DR and regulation of VEGF and endothelial cell function were also characterized. Results We demonstrated that circulating miR-15b was directly associated with VEGF compared with other miRs in patients with PDR. We found a significant inverse relationship between low levels of miR-15b and high levels of VEGF in patients with PDR when compared with the DM or NPDR groups. We found that miR-15b regulates the expression of VEGF by targeting the 3'-untranslated regions to inhibit its transcription. Similarly, overexpression of miR-15b suppressed vascular abnormalities in vivo in diabetic GK rats, inhibiting endothelial tube formation and VEGF expression. Conclusion Circulating miR-15b is associated with PDR and may be targeted to regulate VEGF expression and angiogenesis. Background: Vascular endothelial growth factor (VEGF)-induced angiogenesis is a critical compensatory response to microvascular rarefaction in the diabetic retina that contributes to proliferative diabetic retinopathy (PDR). In this study, we sought to determine the role of specific micro ribonucleic acids (RNAs) (miRs) associated with VEGF in patients with PDR pathology. Methods: RNA sequencing was employed to detect differentially circulating miR associated with VEGF in patients with diabetes mellitus (DM), nonproliferative diabetic retinopathy (NPDR) and PDR. Quantitative real-time polymerase chain reaction was performed to measure the concentration of miR-15b in the serum of patients with DM (n = 115), NPDR (n = 47), or PDR (n = 76). The effects of miR-15b on DR and regulation of VEGF and endothelial cell function were also characterized. Results: We demonstrated that circulating miR-15b was directly associated with VEGF compared with other miRs in patients with PDR. We found a significant inverse relationship between low levels of miR-15b and high levels of VEGF in patients with PDR when compared with the DM or NPDR groups. We found that miR-15b regulates the expression of VEGF by targeting the 3'-untranslated regions to inhibit its transcription. Similarly, overexpression of miR-15b suppressed vascular abnormalities in vivo in diabetic GK rats, inhibiting endothelial tube formation and VEGF expression. Conclusion: Circulating miR-15b is associated with PDR and may be targeted to regulate VEGF expression and angiogenesis. Key Words: diabetic retinopathy, microRNA-15b, proliferative diabetic retinopathy, diabetes, vascular endothelial growth factor, angiogenesis BACKGROUNDVascular endothelial growth factor (VEGF)-induced angiogenesis is a critical compensatory response to microvascular rarefaction in the diabetic retina that contributes to proliferative diabetic retinopathy (PDR). In this study, we sought to determine the role of specific micro ribonucleic acids (RNAs) (miRs) associated with VEGF in patients with PDR pathology. METHODSRNA sequencing was employed to detect differentially circulating miR associated with VEGF in patients with diabetes mellitus (DM), nonproliferative diabetic retinopathy (NPDR) and PDR. Quantitative real-time polymerase chain reaction was performed to measure the concentration of miR-15b in the serum of patients with DM (n = 115), NPDR (n = 47), or PDR (n = 76). The effects of miR-15b on DR and regulation of VEGF and endothelial cell function were also characterized. RESULTSWe demonstrated that circulating miR-15b was directly associated with VEGF compared with other miRs in patients with PDR. We found a significant inverse relationship between low levels of miR-15b and high levels of VEGF in patients with PDR when compared with the DM or NPDR groups. We found that miR-15b regulates the expression of VEGF by targeting the 3'-untranslated regions to inhibit its transcription. Similarly, overexpression of miR-15b suppressed vascular abnormalities in vivo in diabetic GK rats, inhibiting endothelial tube formation and VEGF expression. CONCLUSIONCirculating miR-15b is associated with PDR and may be targeted to regulate VEGF expression and angiogenesis. Abstract Background Vascular endothelial growth factor (VEGF)-induced angiogenesis is a critical compensatory response to microvascular rarefaction in the diabetic retina that contributes to proliferative diabetic retinopathy (PDR). In this study, we sought to determine the role of specific micro ribonucleic acids (RNAs) (miRs) associated with VEGF in patients with PDR pathology. Methods RNA sequencing was employed to detect differentially circulating miR associated with VEGF in patients with diabetes mellitus (DM), nonproliferative diabetic retinopathy (NPDR) and PDR. Quantitative real-time polymerase chain reaction was performed to measure the concentration of miR-15b in the serum of patients with DM (n = 115), NPDR (n = 47), or PDR (n = 76). The effects of miR-15b on DR and regulation of VEGF and endothelial cell function were also characterized. Results We demonstrated that circulating miR-15b was directly associated with VEGF compared with other miRs in patients with PDR. We found a significant inverse relationship between low levels of miR-15b and high levels of VEGF in patients with PDR when compared with the DM or NPDR groups. We found that miR-15b regulates the expression of VEGF by targeting the 3'-untranslated regions to inhibit its transcription. Similarly, overexpression of miR-15b suppressed vascular abnormalities in vivo in diabetic GK rats, inhibiting endothelial tube formation and VEGF expression. Conclusion Circulating miR-15b is associated with PDR and may be targeted to regulate VEGF expression and angiogenesis. Vascular endothelial growth factor (VEGF)-induced angiogenesis is a critical compensatory response to microvascular rarefaction in the diabetic retina that contributes to proliferative diabetic retinopathy (PDR). In this study, we sought to determine the role of specific micro ribonucleic acids (RNAs) (miRs) associated with VEGF in patients with PDR pathology. RNA sequencing was employed to detect differentially circulating miR associated with VEGF in patients with diabetes mellitus (DM), nonproliferative diabetic retinopathy (NPDR) and PDR. Quantitative real-time polymerase chain reaction was performed to measure the concentration of miR-15b in the serum of patients with DM (n = 115), NPDR (n = 47), or PDR (n = 76). The effects of miR-15b on DR and regulation of VEGF and endothelial cell function were also characterized. We demonstrated that circulating miR-15b was directly associated with VEGF compared with other miRs in patients with PDR. We found a significant inverse relationship between low levels of miR-15b and high levels of VEGF in patients with PDR when compared with the DM or NPDR groups. We found that miR-15b regulates the expression of VEGF by targeting the 3'-untranslated regions to inhibit its transcription. Similarly, overexpression of miR-15b suppressed vascular abnormalities in vivo in diabetic GK rats, inhibiting endothelial tube formation and VEGF expression. Circulating miR-15b is associated with PDR and may be targeted to regulate VEGF expression and angiogenesis. |
Audience | Academic |
Author | Yang, Ying Xu, Fan Li, Yiping Adam, Whaley-Connell Yang, Hanling Ylä-Herttuala, Seppo Liu, Yan Chen, Zhongli Yang, Ke Tao, Wenyu Zhou, Taicheng Xiong, Yixin Chaurasia, Shyam S |
AuthorAffiliation | 1 Department of Endocrinology, The Second People’s Hospital of Yunnan Province , Kunming, Yunnan, China 3 Department of Cardiology, Shanghai Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine , Shanghai, China 7 Research Service, Harry S. Truman Memorial Veterans’ Hospital , Columbia, Missouri 6 Division of Endocrinology and Metabolism, Department of Medicine, University of Missouri , Columbia, Missouri 4 Department of Biotechnology and Molecular Medicine, A.I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, Kuopio; and Heart Center and Gene Therapy Unit, Kuopio University Hospital , Finland 2 Institute of Cardiovascular Disease, Ruijin Hospital, Shanghai Jiaotong University School of Medicine , Shanghai, China 5 Ocular Immunology and Angiogenesis Lab, University of Missouri , Columbia, Missouri 8 Division of Nephrology, Department of Medicine, University of Missouri , Columbia, Missouri |
AuthorAffiliation_xml | – name: 5 Ocular Immunology and Angiogenesis Lab, University of Missouri , Columbia, Missouri – name: 7 Research Service, Harry S. Truman Memorial Veterans’ Hospital , Columbia, Missouri – name: 2 Institute of Cardiovascular Disease, Ruijin Hospital, Shanghai Jiaotong University School of Medicine , Shanghai, China – name: 1 Department of Endocrinology, The Second People’s Hospital of Yunnan Province , Kunming, Yunnan, China – name: 3 Department of Cardiology, Shanghai Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine , Shanghai, China – name: 6 Division of Endocrinology and Metabolism, Department of Medicine, University of Missouri , Columbia, Missouri – name: 8 Division of Nephrology, Department of Medicine, University of Missouri , Columbia, Missouri – name: 4 Department of Biotechnology and Molecular Medicine, A.I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, Kuopio; and Heart Center and Gene Therapy Unit, Kuopio University Hospital , Finland |
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Keywords | diabetic retinopathy proliferative diabetic retinopathy diabetes vascular endothelial growth factor angiogenesis microRNA-15b |
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References | Kaikkonen (2021122106050854900_CIT0013) 2017; 10 Ramón (2021122106050854900_CIT0032) 2012; 27 Allen (2021122106050854900_CIT0021) 2019; 60 Shao (2021122106050854900_CIT0011) 2020; 20 Puavilai (2021122106050854900_CIT0016) 1999; 44 Osaadon (2021122106050854900_CIT0002) 2014; 28 Klein (2021122106050854900_CIT0008) 1995; 18 Gong (2021122106050854900_CIT0020) 2016; 59 Tarr (2021122106050854900_CIT0025) 2013; 2013 Wan (2021122106050854900_CIT0009) 2015; 74 Tremolada (2021122106050854900_CIT0004) 2012; 2012 Yang (2021122106050854900_CIT0026) 2010; 235 Zhang (2021122106050854900_CIT0029) 2013; 99 Zhang (2021122106050854900_CIT0034) 2015; 13 Engerman (2021122106050854900_CIT0006) 1986; 35 Haque (2021122106050854900_CIT0014) 2015; 21 Zampetaki (2021122106050854900_CIT0028) 2016; 65 Rabiolo (2021122106050854900_CIT0023) 2015; 56 Jaswani (2021122106050854900_CIT0018) 2017; 6 Liu (2021122106050854900_CIT0030) 2012; 9 Valiatti (2021122106050854900_CIT0024) 2011; 55 Zhou (2021122106050854900_CIT0033) 2016; 7 Gong (2021122106050854900_CIT0010) 2017; 2017 Klein (2021122106050854900_CIT0001) 2007; 14 Bolinger (2021122106050854900_CIT0005) 2016; 17 Li (2021122106050854900_CIT0027) 2017; 37 Chobanian (2021122106050854900_CIT0017) 2003; 42 Guest (2021122106050854900_CIT0022) 2019; 1916 Chan (2021122106050854900_CIT0019) 2013; 86 Nyengaard (2021122106050854900_CIT0007) 2004; 53 Hua (2021122106050854900_CIT0015) 2006; 1 Mohr (2021122106050854900_CIT0012) 2015; 35 Chun (2021122106050854900_CIT0003) 2010; 95 Zheng (2021122106050854900_CIT0031) 2013; 329 Joglekar (2021122106050854900_CIT0035) 2016; 65 |
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Background
Vascular endothelial growth factor (VEGF)-induced angiogenesis is a critical compensatory response to microvascular rarefaction in the... Vascular endothelial growth factor (VEGF)-induced angiogenesis is a critical compensatory response to microvascular rarefaction in the diabetic retina that... Background: Vascular endothelial growth factor (VEGF)-induced angiogenesis is a critical compensatory response to microvascular rarefaction in the diabetic... Background Vascular endothelial growth factor (VEGF)-induced angiogenesis is a critical compensatory response to microvascular rarefaction in the diabetic... BACKGROUNDVascular endothelial growth factor (VEGF)-induced angiogenesis is a critical compensatory response to microvascular rarefaction in the diabetic... |
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SubjectTerms | 3' Untranslated regions Adult Aged Angiogenesis Animals Clinical s Diabetes Diabetes mellitus Diabetic retinopathy Diabetic Retinopathy - metabolism Diabetic Retinopathy - pathology Diabetics Endothelial cells Endothelium Female HeLa Cells Humans Male MicroRNA MicroRNAs - metabolism Microvasculature Middle Aged miRNA Neovascularization, Pathologic - metabolism Neovascularization, Pathologic - pathology Polymerase chain reaction Rats Retina Retina - metabolism Retina - pathology Retinopathy RNA sequencing Transcription Type 2 diabetes Vascular endothelial growth factor Vascular Endothelial Growth Factor A - metabolism |
Title | MicroRNA-15b Targets VEGF and Inhibits Angiogenesis in Proliferative Diabetic Retinopathy |
URI | https://www.ncbi.nlm.nih.gov/pubmed/32797181 https://www.proquest.com/docview/2471030307 https://search.proquest.com/docview/2434480420 https://pubmed.ncbi.nlm.nih.gov/PMC7947967 |
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