Large-Scale Survey of Intraspecific Fitness and Cell Morphology Variation in a Protoploid Yeast Species

• Published in: G3 : genes - genomes - genetics Vol. 6; no. 4; pp. 1063 - 1071
• Main Authors: Jung, Paul P, Sigwalt, Anastasie, Ohnuki, Shinsuke, de Montigny, Jacky, Ohya, Yoshikazu, Schacherer, Joseph
• Format: Journal Article
• Language: English
• Published: United States Genetics Society of America 01.04.2016; Oxford University Press
• Subjects: cell morphology; Cell Shape - genetics; Chromosome Mapping; Cluster Analysis; Energy Metabolism; Environment; fitness; Genetic Association Studies; genetic diversity; Genetic Fitness; Genetic Linkage; Genetic Variation; Investigations; Phenotype; phenotypes; Quantitative Trait, Heritable; Selection, Genetic; Species Specificity; yeast; Yeasts - cytology; Yeasts - genetics; Yeasts - metabolism; genetic diversity; phenotypes; cell morphology; fitness; yeast
• ISSN: 2160-1836; 2160-1836
• DOI: 10.1534/g3.115.026682

It is now clear that the exploration of the genetic and phenotypic diversity of nonmodel species greatly improves our knowledge in biology. In this context, we recently launched a population genomic analysis of the protoploid yeast Lachancea kluyveri (formerly Saccharomyces kluyveri), highlighting a broad genetic diversity (π = 17 × 10(-3)) compared to the yeast model organism, S. cerevisiae (π = 4 × 10(-3)). Here, we sought to generate a comprehensive view of the phenotypic diversity in this species. In total, 27 natural L. kluyveri isolates were subjected to trait profiling using the following independent approaches: (i) analyzing growth in 55 growth conditions and (ii) investigating 501 morphological changes at the cellular level. Despite higher genetic diversity, the fitness variance observed in L. kluyveri is lower than that in S. cerevisiae However, morphological features show an opposite trend. In addition, there is no correlation between the origins (ecological or geographical) of the isolate and the phenotypic patterns, demonstrating that trait variation follows neither population history nor source environment in L. kluyveri Finally, pairwise comparisons between growth rate correlation and genetic diversity show a clear decrease in phenotypic variability linked to genome variation increase, whereas no such a trend was identified for morphological changes. Overall, this study reveals for the first time the phenotypic diversity of a distantly related species to S. cerevisiae Given its genetic properties, L. kluyveri might be useful in further linkage mapping analyses of complex traits, and could ultimately provide a better insight into the evolution of the genotype-phenotype relationship across yeast species.