Glycolipid transfer protein knockout disrupts vesicle trafficking to the plasma membrane

• Published in: The Journal of biological chemistry Vol. 299; no. 4; p. 104607
• Main Authors: Nurmi, Henrik, Backman, Anders P.E., Halin, Josefin, Lönnfors, Max, Blom, Tomas, Roos-Mattjus, Pia, Mattjus, Peter
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.04.2023; American Society for Biochemistry and Molecular Biology
• Subjects: Biological Transport - genetics; Carrier Proteins - genetics; Carrier Proteins - metabolism; Cell Membrane - metabolism; Cell Movement - genetics; Cytosol - metabolism; endoplasmic reticulum (ER); Gene Expression Regulation - genetics; Gene Knockout Techniques; gene silencing; glycerosphingolipid; glycolipid; Golgi; HeLa Cells; Humans; intracellular trafficking; lipid transport; Protein Binding - genetics; sphingolipid; VAP-A; VSVG; GLTP; glycolipid; VAP-A; VAP; sphingolipid; VSVG; gene silencing; lipid transport; GO; intracellular trafficking; glycerosphingolipid; ER; gRNA; DMEM; GSLs; Golgi; cDNA; endoplasmic reticulum (ER); ERES; MCS; OCR

The glycolipid transfer protein (GLTP) has been linked to many cellular processes aside from its best-known in vitro function as a lipid transport protein. It has been proposed to act as a sensor and regulator of glycosphingolipid homeostasis in cells. Furthermore, through its previously determined...