The sonication-assisted whisker method enables CRISPR-Cas9 ribonucleoprotein delivery to induce genome editing in rice

CRISPR/Cas9-based genome editing represents an unprecedented potential for plant breeding. Unlike animal cells, plant cells contain a rigid cell wall, genome editing tool delivery into plant cells is thus challenging. In particular, the delivery of the Cas9-gRNA ribonucleoprotein (RNP) into plant ce...

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Published inScientific reports Vol. 13; no. 1; p. 14205
Main Authors Nakamura, Akiyoshi, Yano, Tsubasa, Mitsuda, Nobutaka, Furubayashi, Maiko, Ito, Seiichiro, Sugano, Shigeo S., Terakawa, Teruhiko
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 07.09.2023
Nature Publishing Group
Nature Portfolio
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